The Spillover Spreading Matrix (SSM) is a measure of the performance of an instrument for a given panel.  It reflects the Poisson-based error in fluorescence quantification.  SSM’s can be calculated for a set of single stain controls in the Compensation Editor. This tool works for matrices created in FlowJo, as the single stain controls are… Read more »

Updates Search bar added to parameter selectors in the graph window. Color mapped (aka “heat map statistic”) parameter labeling in layouts. Stopped Compensation Wizard matrix re-calculations (ad infinitum). Workspace Title will no longer automatically revert to “unsaved” immediately after a save. Special considerations for Cytek data scaling implemented. Color Mapping axis keyboard shortcuts are now functional…. Read more »

New Features Search bar added to parameter selectors in the graph window. Color mapped (aka “heat map statistic”) parameter labeling in layouts. Bugs Fixed Proliferation Platform Improvements Stopped Compensation Wizard matrix re-calculations (ad infinitum). More Info For more details on features in this release, and a comprehensive list of known issues visit the exhaustive release notes…. Read more »

Color mapping in FlowJo’s graph window allows users to visualize a third parameter in the two-dimensional display, by illustrating a Median value for any tertiary parameter in a color scale applied to the dots displayed. Accessing the Color Map Checking the box for “Color Axis” will display the expression levels of a third parameter represented… Read more »

Below are the exhaustive release notes and known issues. For the general release notes, please see this page. Updates FlowJo Enterprise (deprecated) button now replaced with a FlowJo Envoy Integration Help button. Updated iconography throughout. Updated Java Runtime Environment – version 1.8.0_144. Known Issues Proliferation: This feature may fail to correctly model certain data files. This… Read more »

New Features: Hotly anticipated color mapping within the graph window, to illustrate a third parameter there is now available. Spillover spreading matrix (SSM) now available in the Compensation Editor. Warnings: When saving an ACS file from a FlowJo Envoy based workspace, the default location prompt is a temporary cache folder, which will be deleted when FlowJo… Read more »

BD Research Cloud is now integrated with FlowJo v10.8! BD Research Cloud is an application for communication and collaboration across a lab’s entire toolbox, including but not limited to flow cytometry and sequencing processes. Being integrated directly into the FlowJo workspace means you can access any analyses and data-sets shared with you in BD Research… Read more »

New Features: We are happy to announce that the Proliferation Platform is here! We have revised the Logicle Transform implementation. Existing workspaces with gates using this transform may need to be adjusted. Warnings: Because FlowJo® now uses Java 8, we no longer support Mac OS 10.6.8 (because it does not support Java 8). Major Changes:… Read more »

Below are the exhaustive release notes and known issues. For the general release notes, please see this page. Known Issues Compensation: A BD 25-color data file may not include the compensation matrix when exported from and brought back into FlowJo. Workaround: Export the compensation matrix as an MTX file, and re-apply it to the exported data… Read more »

New Features Note:  This version of FlowJo is compatible with FlowJo Enterprise v2.0 or the newly released FlowJo Enterprise 2.1. Be sure your version of FlowJo Enterprise is new enough before installing this version of FlowJo. New FlowJo Plugin Architecture.   FlowJo can now use plugins that can perform a variety of functions, including the creation… Read more »