Below are the exhaustive release notes and known issues. For the general release notes, please see this page. Updates FlowJo Enterprise (deprecated) button now replaced with a FlowJo Envoy Integration Help button. Updated iconography throughout. Updated Java Runtime Environment – version 1.8.0_144. Known Issues Proliferation: This feature may fail to correctly model certain data files. This… Read more »

New Features: Hotly anticipated color mapping within the graph window, to illustrate a third parameter there is now available. Spillover spreading matrix (SSM) now available in the Compensation Editor. Warnings: When saving an ACS file from a FlowJo Envoy based workspace, the default location prompt is a temporary cache folder, which will be deleted when FlowJo… Read more »

BD Research Cloud is now integrated with FlowJo v10.8! BD Research Cloud is an application for communication and collaboration across a lab’s entire toolbox, including but not limited to flow cytometry and sequencing processes. Being integrated directly into the FlowJo workspace means you can access any analyses and data-sets shared with you in BD Research… Read more »

New Features: We are happy to announce that the Proliferation Platform is here! We have revised the Logicle Transform implementation. Existing workspaces with gates using this transform may need to be adjusted. Warnings: Because FlowJo® now uses Java 8, we no longer support Mac OS 10.6.8 (because it does not support Java 8). Major Changes:… Read more »

Below are the exhaustive release notes and known issues. For the general release notes, please see this page. Known Issues Compensation: A BD 25-color data file may not include the compensation matrix when exported from and brought back into FlowJo. Workaround: Export the compensation matrix as an MTX file, and re-apply it to the exported data… Read more »

New Features Note:  This version of FlowJo is compatible with FlowJo Enterprise v2.0 or the newly released FlowJo Enterprise 2.1. Be sure your version of FlowJo Enterprise is new enough before installing this version of FlowJo. New FlowJo Plugin Architecture.   FlowJo can now use plugins that can perform a variety of functions, including the creation… Read more »

Below are the exhaustive release notes and known issues.  For a digest of these release notes, please see this page. Bugs Fixed Improved the file saving behavior with ACS files Fixed an issue where very large and complex workspaces could take a very long time to close. (A side effect of this is that you… Read more »

New Features Note:  This version of FlowJo is NOT compatible with FlowJo Enterprise. Stay tuned for the upcoming FlowJo Enterprise release that will work with this version. New FlowJo Plugin Architecture.   FlowJo can now use plugins that can perform a variety of functions, including the creation of populations, creation of derived parameters, and actions when… Read more »

T-Distributed Stochastic Neighbor Embedding (tSNE) is an algorithm for performing dimensionality reduction, allowing visualization of complex multi-dimensional data in fewer dimensions while still maintaining the structure of the data. tSNE is an unsupervised nonlinear dimensionality reduction algorithm useful for visualizing high dimensional flow or mass cytometry data sets in a dimension-reduced data space.  The tSNE platform computes two new… Read more »

Plugins are executable java files that extend functionality of the FlowJo application. These can be installed and used as shown below. Installing R on a Windows PC: Installing R on a Mac: Installing the PluginPlay Bundle: Installing Plugins in FlowJo v10: Create a folder named “plugins” on your computer. On a Windows computer this folder… Read more »