Deriving a Kinetics Parameter

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You can use the derived parameter platform to create ratios of parameters for use in the kinetics platform Some data files intended for kinetics analysis will contain parameters of the individual measures of bound and free calcium without containing the ratio parameter that is commonly used to measure cellular changes.  In this case the derived… Read more »

10.0.8 Exhaustive Release Notes

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Below are the exhaustive release notes and known issues.  For a digest of these release notes, please see this page. New Features: Cell Cycle: New 1d Cell Cycle Platform with both Watson Pragmatic and Dean Jett Fox modeling. Export: Completely revised, Export/Concatenate now has all the features you loved in v9 Kinetics: New implementation of… Read more »

10.0.8 Release notes

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You’ll notice some major improvements in this 10.0.8 release, from the very start of the application through the interface to new platforms.  In terms of user feedback, features, quality, and performance, this represents a major release and we are grateful for the many conversations with loyal users and work with beginning users whose feedback help… Read more »

Workspace XML

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An entire FlowJo v10   workspace is described by XML. You can view a workspace’s XML to reveal all that is contained within it. That is, gate dimensions, positions of vertices, file and node names, gating trees, statistics, layouts, plates, tables and formulas are all recorded for each FlowJo v10  workspace. FlowJo v10  also provides a… Read more »

Activity Log

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FlowJo v10 maintains an activity log that records every modification to a workspace, template, or ACS file. Need to know exactly how and where a modification was performed or how long a workspace was open? These details are recorded in FlowJo v10’s ActionLog viewer. You can view the activity log’s XML directly by navigating to the Power… Read more »

Using a Proxy

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A proxy server is a server that acts as an intermediary between computers seeking resources from other servers.  Typically these are used to add a layer of security to a network by screening what goes in and out of a network. If your institution uses a proxy server and a FlowJo site license you will… Read more »

Replacing Nodes

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When you drag a node onto another sample, FlowJo checks to see if the name of the destination node already has a gate with the same name of the node that you are dragging. If the name of the destination gate matches the name of the gate you are dragging then the node replacement dialog… Read more »

Image Files in FlowJo

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Working with images? You can open, gate, and add statistics to image files with the latest release of FlowJo v10 (10.0.7 or later). Image files, like FCS files, can be parsed into component parts for analysis. Similar to events or cells in an FCS file, pixels are characterized by their color and brightness attributes. In this… Read more »

R-Tools in FlowJo

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is an open source programming environment used by scientists to create software tools for their research needs.  Many of these tools have been shared and can be incorporated at no cost into your cytometric data analysis workflow. There are three ways that you can use R with FlowJo, listed here in order of increasing computer… Read more »

Templates and Compensation

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Applying new compensation matrices to data loaded into templates can be a bit confusing. This document will help! When a template is created on compensated parameters, and new data is loaded that was compensated on the machine (includes an acquisition matrix), these data will load right into the template and gates will be applied. However,… Read more »