Preferences in FlowJo are set by default to rescale the data based on our understanding of the differences between SONY’s acquisition display and the written format of the file.  FlowJo’s default preferences should display the data the same way as seen on the instrument. You can change these default preference sets in the cytometer specific preferences…. Read more »

The Plate Editor Experiment Band facilitates experiment-level annotation as well as the movement of keyword-value pairs from workspace groups to the plate and vice versa.   Annotate Experiment Brings up the MIFlowCyt annotation tool allowing you to add experimental details.   Read Attributes from Group Assigns the keywords from the data files in the currently selected group… Read more »

The Settings Band allows visualization of the number of attributes added to a well, numerical keys, and categories of wells through the use of borders in FlowJo’s Plate Editor. The Plate Editor: Settings Band   Show number of attributes Puts an indicator of the number of keyword-value pairs for each well in the bottom right-hand corner… Read more »

The Configure Band allows a user to set plate dimensions, annotate wells with a dilution series, and add/remove staging wells in FlowJo’s Plate Editor.   Set Plate Dimensions Allows the user to define the plate layout (number of wells) and change between 96, 384, and 1536 well plates. Dilution Series Set up a titration keyword… Read more »

The Document band in the Plate Editor allows the user to save or open plate setup XML files or export and import CSV files for plate annotation.  It also contains functions for exporting the plate setup as a template for annotating future plate or DiVa acquisition file. Open… Loads a saved plate setup (XML) file which contains previously… Read more »

The Plate Editor Keywords band allows the addition and deletion of annotation keywords within the plate, as well as the ability to import keyword-value pairs from a CSV spreadsheet file. Add Keywords From File… Adds keyword-value pairs from a comma-separated values (.csv) spreadsheet file, to the “Drag to Wells” list, where they can then be applied… Read more »

The Select Band in the Plate Editor allows editing and saving of well selections, making groups from selected wells, and filling in annotation in a plate. Select All Selects all of the wells in the current plate, so that adding a keyword-value pair to one well adds it to the whole plate. Deselect All Deselects all of… Read more »

The Clipboard band in the Plate Editor contains functions that allow the user to duplicate or remove annotations from the plate. The most recently cut or copied item is saved to the clipboard and can be inserted back into the plate using the Paste function. Cut The Clipboard band contains functions that allow the user to duplicate… Read more »

The Plate band located in the plate editor contains a list of plates created by the user. From this band the user can choose which plate to view, delete plates, and create new plates. Rename a Plate by double-clicking a table in the list, a user may rename a plate. New (+) Creates a new, blank… Read more »

The FlowJo Plate Editor Ribbon is customizable to give you quick access to frequently used tools. The Ribbon contains a set of tabs and bands, along with access buttons to change your preferences and ribbon configuration. The bands within the Plate Editor that can be added or customized are shown below – For more information on these bands and… Read more »