General FAQ for installation, licensing, and pricing questions can be found in this document. For technical FAQ, please go here. 1) How do I install FlowJo? 2) How do I locate the hardware address (HWA) for FlowJo licensing and 30 day trials? 3) What are the different licensing options available? 4) What is the price… Read more »

FlowJo v10 has a number of performance settings to help you utilize more RAM, more processing cores, and additional hardware available for your specific computer. The preference settings are described here. Some additional discussion points are address on this daily dongle post.      

Asking this questions means that we need to address the cause as well. Therefore, please view this page. Scroll down to the section “FlowJo-FCS-Files Rules to Live By” and start reading. Understanding the way FlowJo works with and stores your FCS data will save you headaches later!

In order to change the preferences, just go into the preference interface and set any preference you’d like. For more on preferences and how to access them, click here. *Note: Not all preferences will take effect immediately. Many require a restart of the program and possibly reanalysis.  For example, changing the gate line thickness will… Read more »

Double click on the legend in the layout editor to open the interface that will provide options to edit. All the features available in legends are provided here. If you want to specifically add statistics, view the bottom of the page linked above.

Overlays are done in the layout editor. Simply drag a sample into the layout editor and then drag another sample or population from the workspace on top of an existing graph in the layout editor. For more information, click here.

The scale of the parameter can be altered using the T button in the graph window. Updates to scaling and transformation propagate to the image in the layout editor as well. For more on the T button and how to change scaling, please go here. Scaling preferences exist in the general program preferences as well…. Read more »

To add a statistic or statistics to samples in the workspace, open the statistic interface. Information on statistics can be found here.

To place plots into the layout editor, just drag and drop samples or populations from the workspace into the layout editor window. For more details, please go here.

To add statistics to the Table Editor, just drag and drop them from the workspace into the table editor interface. This document explains the process in greater detail.