Populations Band

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This band provides the opportunity to create new gates, edit sample annotations, view metadata, and rename populations. Rename Clicking this button allows you to change the given name of the selected population. tSNE Create a T-Distributed Stochastic Neighbor Embedding (tSNE) plots. tSNE is an unsupervised nonlinear dimensionality reduction algorithm useful for visualizing high dimensional flow… Read more »

Select Band

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The Select band is located under the Edit tab of your Workspace. This band contains three icons that provide sample and population node selection options as described below. Select Samples Clicking Select Samples reveals a dropdown menu which allows you to select an alternating sequence of samples beginning with the currently selected sample. For example,… Read more »

Settings Band

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The Settings band allows the user to customize his or her personal configuration of FlowJo. Here the user can adjust the width and content of columns displayed in the workspace, and show or hide statistics. Edit Columns Clicking this icon opens a dialog box that allows the user to add or remove keyword columns to… Read more »

Statistics Band

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The Statistics band makes it easy to add statistical information about the selected sample to the Workspace. When you select a sample and use one of the functions on this band, FlowJo computes the desired statistic for you and displays it beneath the chosen sample in the workspace. Add Statistic Clicking this icon opens up… Read more »

Expand Band

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Selecting an Expand/Collapse icon provides four actions to manipulate the display of a Workspace by expanding or collapsing the analysis hierarchy of a particular sample or a group of samples. These functions reduce scrolling when you are analyzing a large number of samples. Expand Selection Reveals any hidden items in the analysis hierarchy of the selected population… Read more »

Find Band

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There are two action icons located within the Find band that allow you to search for FCS data files or text within these data files. These actions can help you quickly locate and/or replace keywords and files from your Workspace. Find Search for text in keywords of the data files. FCS Scan Process your local… Read more »

Navigate Band

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From the FlowJo Navigate band, users can open FlowJo’s Table Editor and Layout Editor. This band also allows the user to create a new workspace, access preferences, add a new group to the existing workspace, or browse the file system for samples (in .FCS format) to add to the current workspace. New Workspace Clicking this icon will… Read more »

Groups Band

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The Groups band contains functions that allow the user to create and manipulate groups from the samples in the workspace. From here the user can group selected samples together, create groups based on a certain keyword (i.e. a group of all samples with the same Patient ID), or copy specific values or gates to existing… Read more »

Help Band

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The Help band contains information about using FlowJo. From this band the user can check version info, access tutorials, user manuals, email flowjosupport@bd.com and link to the official FlowJo blog. Workspace Links About Clicking the About icon opens a dropdown menu with two options. The “About” button opens a window that contains version, engine, Java,… Read more »

Cytometry Band

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The Cytometry band allows users to access additional platforms required for specific analyses. Compensation Compensation will provide an interface to identify single stain controls, set proper gates and create the compensation matrix.  This process is fundamental to accurately analyzing multicolor immunofluorescence flow cytometry data. This button in the workspace allows access the compensation editor interface, where… Read more »