Right-clicking on a file in the workspace or a sub-population includes an option for automatically creating bisector gates. First, why “BifurGate?” Bifurcate is a verb which means “to divide into two branches or forks,” and a gate is a selected subpopulation.  Putting these works together gets us BifurGate, which will divide populations into two gates… Read more »

I downloaded FlowJo v10 now what?! Get started with some free tutorials, including demo data and learn how to do your analysis in just a few clicks.   The tutorial’s demo data contains a set of FCS raw data and a detailed printable instruction manual (PDF, DOC, or TXT file).  Don’t have time to read the manuals? No… Read more »

The ever-popular pseudocolor plot is back again in FlowJo v10! Extract more relevant information from your graphical display, get a “feel” for your data, and wow your co-workers with a colorful display of your experimental results. Pseudocolor plots are a type of bivariate density plot. That is, they display the relative population density of cell… Read more »

Guides are a simple way to align objects’ edges in the Layout Editor. Under the Edit Tab, in the Show & Hide Band, is a toggle button to Show Guides. To create new Guides, drag either ruler into the layout.  Objects that are dragged in the vicinity of a Guide will appear to snap to it… Read more »

The right side of the Layout Editor contains a sidebar of extra options called Properties. There is a copy of the Layouts Band (Layouts tab), convenient for those who need more than a handful of layouts in the experiment and don’t want to go back to the Layout Editor Ribbon. There is a copy of… Read more »

The tool to assist in rapid visualization of plate-based flow data statistics is located in the Layout Editor. Open the Layout Editor and select the Plate tool. A click, drag and release movement with this tool in the layout editor will create a single open cell. Within the workspace, select the group you wish to… Read more »

Keywords, or columns of keywords, can be copied from a spreadsheet and pasted into a FlowJo Workspace. To add a spreadsheet list as keywords to the Workspace: From your spreadsheet program, copy the column of values you would like to add to the Workspace, click on the first cell in the Workspace and use Ctrl+V to… Read more »

This band provides the opportunity to create new gates, edit sample annotations, view metadata, and rename populations. Rename Clicking this button allows you to change the given name of the selected population. tSNE Create a T-Distributed Stochastic Neighbor Embedding (tSNE) plots. tSNE is an unsupervised nonlinear dimensionality reduction algorithm useful for visualizing high dimensional flow… Read more »

The Select band is located under the Edit tab of your Workspace. This band contains three icons that provide sample and population node selection options as described below. Select Samples Clicking Select Samples reveals a dropdown menu which allows you to select an alternating sequence of samples beginning with the currently selected sample. For example,… Read more »

The Settings band allows the user to customize his or her personal configuration of FlowJo. Here the user can adjust the width and content of columns displayed in the workspace, and show or hide statistics. Edit Columns Clicking this icon opens a dialog box that allows the user to add or remove keyword columns to… Read more »