From the FlowJo Navigate band, users can open FlowJo’s Table Editor and Layout Editor. This band also allows the user to create a new workspace, access preferences, add a new group to the existing workspace, or browse the file system for samples (in .FCS format) to add to the current workspace. New Workspace Clicking this icon will… Read more »

The Groups band contains functions that allow the user to create and manipulate groups from the samples in the workspace. From here the user can group selected samples together, create groups based on a certain keyword (i.e. a group of all samples with the same Patient ID), or copy specific values or gates to existing… Read more »

The Help band contains information about using FlowJo. From this band the user can check version info, access tutorials, user manuals, email flowjosupport@bd.com and link to the official FlowJo blog. Workspace Links Workspace links contains a set of links to this help documentation regarding the workspace. About Clicking the About icon opens a dropdown menu… Read more »

The Cytometry band allows users to access additional platforms required for specific analyses. Compensation Compensation will provide an interface to identify single stain controls, set proper gates and create the compensation matrix.  This process is fundamental to accurately analyzing multicolor immunofluorescence flow cytometry data. This button in the workspace allows access the compensation editor interface, where… Read more »

Keywords used in the FlowJo workspace, are valuable annotation embedded in FCS data files. You can utilize them to sort and filter samples based on their annotation characteristics. These keywords are combined with groups to effectively subset analyses into components. Add Keyword Clicking this icon allows the user to create a new attribute/value pair for… Read more »

FlowJo’s Biology Band contains a series of tools for applying mathematical models to specific biological systems. Cell Cycle The cell cycle platform creates a one-dimensional model of DNA content for the selected population using either the Watson or Dean-Jett-Fox models. To use this tool, click on a population at any level of the gating hierarchy… Read more »

The Boolean gates ribbon contains a series of tools for using existing gates to create additional, more complex gates. Create Combination Gates Create Combination Gates makes a set of Boolean gates comprised of all possible combinations of the gates selected at the time this function is chosen. For example, if you have gates “a”, “b” and… Read more »

The Clipboard band contains functions that allow the user to duplicate or remove samples, statistics, or pieces of text from the Workspace. The most recently cut or copied item is saved to the clipboard and can be inserted back into the workspace using the Paste function. Cut The Clipboard band contains functions that allow the… Read more »

The Power band provides access to diagnostic and benchmarking functions. This should not be required for routine use of the program, but is helpful if attempting to resolve problematic cases.  Diagnostics Contains access points to: Script editor Allows users to code additional functionality into FlowJo within the program.  Uses Java Script.

The Document band allows the user to save changes to the workspace, revert to the last save state, or export the workspace to the user’s hard drive. It also contains functions for renaming and inspecting selected samples from the workspace and opening the graph windows for those samples. Print Clicking this icon opens a dialog… Read more »