Below are instructions for granting “Full Disk Access” to FlowJo on MacOS’s Catalina, Big Sur and Monterey for proper Dongle detection. Text-only Instructions:1. Open up your MacOS System Preferences and select Security & Privacy2. From the Security & Privacy window, select “Full Disk Access” and click the lock at the bottom left corner to unlock… Read more »
MacOS13 Permissions (Ventura)
Below are instructions for granting “Full Disk Access” to FlowJo on MacOS’s Catalina, Big Sur and Monterey for proper Dongle detection. Please note that due to a change in MacOS, Dongles will not authenticate FlowJo 10.8.1 or older versions, on MacOS Ventura. We released an updated build, FlowJo v10.8.2, which addresses this issue and supports Dongle authentication on… Read more »
Finding Dongle ID (Legacy Black Dongles <2009)
Dongle Serial ID Lookup Instructions Prior to 2009 is an older version of the all black capped dongle that allowed access to version 7/8. These are the instructions for legacy black Dongles delivered prior to 2009: Mac OS Text-only Instructions: 1. Click on Apple icon (top left of screen)2. Select About This Mac3. Click on… Read more »
Finding Dongle ID (Older Dongles – Red/Black Switchblade 2009-2023)
Dongle Serial ID Lookup Instructions Windows OS Instructions for older Dongles delivered prior to March 2023 Text-only Instructions: Full Instructions with Images: 5. Click the Hardware tab 6. Under All disk drives, select STT USB_JDTT USB Device 7. Press the Properties button on the bottom of the window 8. Click on the Details tab 9. In the Property dropdown, choose Device instance path 10…. Read more »
Finding Dongle ID (Newer Dongles – Black with cap – Delivered starting March 2023)
Dongle Serial ID Lookup Instructions Windows OS Instructions for newer Dongles delivered starting March 2023 Text-only Instructions: Full Instructions with Images: 5. Click the Hardware tab 6. Under All disk drives, select STT USB_JDTT USB Device 7. Press the Properties button on the bottom of the window 8. Click on the Details tab 9. In the Property dropdown, choose Device instance path 10. Copy… Read more »
FlowJo Dongle Licensing
The FlowJo dongle has been a longstanding license type utilized by users to access FlowJo. A dongle is a USB loaded with a license.key file that grants access to the FlowJo desktop application. A user can plug their Dongle into a computer and open a fully authenticated FlowJo session. Dongles can be used by multiple… Read more »
Rosetta Calibration
Understanding the size of particles in flow cytometry is important in a number of applications. FlowJo™ Software with the Rosetta Calibration plugin, along with the Rosetta Calibration product from Exometry enables calibrated size measurements of small spherical particles (<10 μm). The plugin allows you to utilize FCS data captured from Rosetta Calibration beads to derive… Read more »
IndexSort
Introduction This plugin was designed in order to give researchers the ability to automatically gate wells from .fcs data generated using the BD FACSAriaTM, the BD FACSAriaIITM, the BD FACSAriaIIITM, the BD FACSAria FusionTM, the BD FACSMelodyTM, the BD Influx SystemTM, the BD FACSJazzTM, FACSVulcanTM, and the BD FACSymphonyTM S6 cell sorters. Please review FlowJo… Read more »
Cluster Explorer
IntroductionCluster Explorer is a FlowJo plugin. The tool creates an interactive cluster Profile graph, heatmap, and displays the cluster populations on a tSNE/UMAP plot. The plots are dynamic, can be copied to the clipboard or FlowJo Layout, and allow the user to select populations in one view and highlight the selected population in the other… Read more »
UMAP
How-to-UMAP Uniform Manifold Approximation and Projection (UMAP) is a machine learning algorithm used for dimensionality reduction to visualize high parameter datasets in a two dimensional space. (1) The UMAP plugin will run with both FlowJo and SeqGeq bioinformatics data analysis platforms. Note: The UMAP plugin for FlowJo requires requires a 64bit OS. This is due… Read more »