10.6 Exhaustive Release Notes

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Updates Spectral Compensation option available for compensating appropriately collected data within the Compensation Wizard. Platform overlays for: Kinetics, Cell-Cycle, and Proliferation now available within the Layout Editor. Auto-Save workspace feature – new preference setting allows researchers to automatically save their analysis at a customizable time interval. This popular feature has been requested by many researchers, and we heard… Read more »

10.6 Release Notes

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New Features: Spectral Compensation now available for compensating data collected with an overdetermined system (more detectors than fluorescent probes), within the Compensation Wizard. Figures generated by Platforms in FlowJo v10 can now be overlaid! Yes, you read that correctly, you can now overlay: Kinetics, Cell-Cycle, and Proliferation platform figures within the Layout Editor in FlowJo…. Read more »

Diva Integration

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Since 1994, Flow Cytometrists have been using FlowJo to analyze data collected on BD cytometers to create statistics and figures pivotal to publications on immune subsets. Until now, FCS files were the only data from FACSDiva™ that could be imported into FlowJo. Now, with FlowJo v10.6 and newer, FACSDiva™ experiments can be imported into FlowJo… Read more »

Platform Overlays

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Starting in FlowJo v10.6, platform figures (Kinetics, Cell Cycle, and Proliferation) can be overlaid in the Layout Editor by dragging and dropping the platform nodes on top of one another. This greatly aids in the comparison of results produced by the various modeling platforms, and makes for a killer graph to show at your next lab… Read more »

Multigraph Color Mapping

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Creating multiple color maps for a single bivariate plot is often useful in analyzing high parameter datasets. This process used to be quite tedious and time consuming – no longer! In FlowJo v10.6, researchers now have the option to create a multigraph color-map in just a few clicks. In the Layout Editor, simply right click… Read more »

Auto-Save

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Back by popular demand, Auto-Save is now available in FlowJo v10.6 and newer. By default FlowJo v10 will not automatically save workspaces. To turn this functionality on, simply visit the Workspace section of the preferences and choose a time frame for the auto-save to cycle (in minutes). This will keep saving the workspace, automatically, wherever… Read more »

Spectral Compensation

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In FlowJo v10.6 there is now a check-box labelled Spectral at the top of the compensation wizard to enable spectral compensation functionality. Alongside that check-box are options to choose weights per fluorescence detector and to optimize the weighting used. To utilize spectral compensation, you will first need data that contains more detectors than fluorescent probes (an overdetermined system)… Read more »

Auto-generate Custom Table Column Headers

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Within the Table Editor there is a Name column that allows researchers to enter a customized header for columns added there. A new feature introduced in FlowJo v10.6 or later allows researchers to set a custom combination of values to auto-name table columns. When creating a table from the Table Editor, simply choose the Edit… Read more »

10.5.3 Exhaustive Release Notes

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Updates New native tSNE platform. Note: This does not replace the legacy tSNE plugin (as that will be required as an object within FlowJo’s plugin folder) but is a preferable method of calculating derived parameters, due to its supremely improved performance. Derived parameter fixes. Memory management issues fixed. Native tSNE now runs correctly on grandchild… Read more »

10.5.3 Release Notes

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New Features: New tSNE Platform now offers super high performance dimensionality reduction. Warnings: For tSNE mappings in the latest release, parameters should not be scaled using log or lin transformations. We generally recommend using Biex transformations for those parameters. Log and lin transformed parameters can lead to over-clustering within the tSNE embedded space. Security protocols… Read more »