10.0.7 Exhaustive Release Notes

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For a digest of these release notes, please see this page.  This page contains an exhaustive list of issues that have been reported and fixed, new features implemented, and known issues. Fixed:   FlowJo 10.0.7r2: Significant Improvements: Compensation is now “remembered” in templates Compensation population size restriction has been reduced to a warning if under… Read more »

Txt2FCS

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Text to FCS (Txt2FCS) Back to Utilities HQ Text To FCS is a utility plugin that ships with FlowJo to allow dragging of CSV and Image files directly into FlowJo. Since it is included in FlowJo no additional download or installation is necessary. CSV files support a header row with parameter names followed by numerical-only… Read more »

DDU (Data Deidentification Utility)

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Data Deidentification Utility Back to Utilities HQ   Data Deidentification Utility scrubs FCS data’s keyword values. It supports batching a large selection of FCS files and features an easy interface for selecting which keyword values to scrub. Click here to download the JAR.      

The FlowJo Diagnostic

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The FlowJo Diagnostic Back to Utilities HQ FlowJo Diagnostics is a set of macros which ships with FlowJo installers for the Windows computers. It is designed to detect and report low level problems in areas such as internet connectivity, file corruption, invalid installation and poor performance (system tuning and benchmarking.) The Diagnostic is also a… Read more »

CommanJo

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CommanJo Back to Utilities HQ FlowJo Commandline is an architecture within the FlowJo software intended for “hands free” processing. The syntax and creation of these commands can be laborious and error prone – tabs, spaces, quotes can make or break the execution. To reduce the uncertainties, using CommanJo allows you to compose these with a… Read more »

Utilities

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Welcome to the FlowJo Utilities section of the documentation. CommanJo – commanjo is a “control panel” for composing and executing FlowJo command line FJDiagnostic – this utility ships with FlowJo for Windows and helps diagnose and benchmark FlowJo. Txt2FCS – written originally to support “non-flow” sources of data, now integrated into the program to natively support CSV and basic image… Read more »

Cell Cycle: Univariate

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Univariate modeling can be used to create a fit to cell cycle data based on statistics in one dimension, traditionally DNA content. FlowJo provides a simple interface to performing fairly sophisticated DNA/Cell Cycle analysis.  To launch the univariate cell cycle model click on the population of interest in the workspace, then select the Cell Cycle task from… Read more »

SP6800 Spectral Analyzer

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The SP6800 Spectral Analyzer uses software, developed by Sony, which performs spectral unmixing and exports FCS files for analysis in FlowJo. Spectral analyzers collect all emitted florescence simultaneously, then use spectral unmixing to separate the individual colors.  Spectral unmixing is the process in which a set of controls that represent each fluorescent color individually, similar to… Read more »

EC800 Software

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Preferences in FlowJo are set by default to rescale the data based on our understanding of the differences between SONY’s acquisition display and the written format of the file.  FlowJo’s default preferences should display the data the same way as seen on the instrument. You can change these default preference sets in the cytometer specific preferences…. Read more »

Experiment Band

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The Plate Editor Experiment Band facilitates experiment-level annotation as well as the movement of keyword-value pairs from workspace groups to the plate and vice versa.   Annotate Experiment Brings up the MIFlowCyt annotation tool allowing you to add experimental details.   Read Attributes from Group Assigns the keywords from the data files in the currently selected group… Read more »