Data De-identification Utility

Tags:

A standalone tool for de-identifying FCS files. The Data De-identification Utility scrubs FCS data’s keyword values. It supports batching a large selection of FCS files and features an easy interface for selecting which keyword values to scrub. Click here to download the tool and How-to document. NOTE: The de-identification utility is not a commercially sold… Read more »

Cell Cycle: Univariate

Tags:

Univariate modeling can be used to create a fit to cell cycle data based on statistics in one dimension, traditionally DNA content. FlowJo provides a simple interface to performing fairly sophisticated DNA/Cell Cycle analysis.  To launch the univariate cell cycle model click on the population of interest in the workspace, then select the Cell Cycle task from… Read more »

SP6800 Spectral Analyzer

Tags:

The SP6800 Spectral Analyzer uses software, developed by Sony, which performs spectral unmixing and exports FCS files for analysis in FlowJo. Spectral analyzers collect all emitted florescence simultaneously, then use spectral unmixing to separate the individual colors.  Spectral unmixing is the process in which a set of controls that represent each fluorescent color individually, similar to… Read more »

EC800 Software

Tags:

Preferences in FlowJo are set by default to rescale the data based on our understanding of the differences between SONY’s acquisition display and the written format of the file.  FlowJo’s default preferences should display the data the same way as seen on the instrument. You can change these default preference sets in the cytometer specific preferences…. Read more »

Experiment Band

Tags:

The Plate Editor Experiment Band facilitates experiment-level annotation as well as the movement of keyword-value pairs from workspace groups to the plate and vice versa.   Annotate Experiment Brings up the MIFlowCyt annotation tool allowing you to add experimental details.   Read Attributes from Group Assigns the keywords from the data files in the currently selected group… Read more »

Settings Band

Tags:

The Settings Band allows visualization of the number of attributes added to a well, numerical keys, and categories of wells through the use of borders in FlowJo’s Plate Editor. The Plate Editor: Settings Band   Show number of attributes Puts an indicator of the number of keyword-value pairs for each well in the bottom right-hand corner… Read more »

Configure Band

Tags:

The Configure Band allows a user to set plate dimensions, annotate wells with a dilution series, and add/remove staging wells in FlowJo’s Plate Editor.   Set Plate Dimensions Allows the user to define the plate layout (number of wells) and change between 96, 384, and 1536 well plates. Dilution Series Set up a titration keyword… Read more »

Document Band

Tags:

The Document band in the Plate Editor allows the user to save or open plate setup XML files or export and import CSV files for plate annotation.  It also contains functions for exporting the plate setup as a template for annotating future plate or DiVa acquisition file. Open… Loads a saved plate setup (XML) file which contains previously… Read more »

Keywords Band

Tags:

The Plate Editor Keywords band allows the addition and deletion of annotation keywords within the plate, as well as the ability to import keyword-value pairs from a CSV spreadsheet file. Add Keywords From File… Adds keyword-value pairs from a comma-separated values (.csv) spreadsheet file, to the “Drag to Wells” list, where they can then be applied… Read more »

Select Band

Tags:

The Select Band in the Plate Editor allows editing and saving of well selections, making groups from selected wells, and filling in annotation in a plate. Select All Selects all of the wells in the current plate, so that adding a keyword-value pair to one well adds it to the whole plate. Deselect All Deselects all of… Read more »