Overlay Populations

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Overlays are done in the layout editor. Simply drag a sample into the layout editor and then drag another sample or population from the workspace on top of an existing graph in the layout editor. For more information, click here.

Adjust the Scale

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The scale of the parameter can be altered using the T button in the graph window. Updates to scaling and transformation propagate to the image in the layout editor as well. For more on the T button and how to change scaling, please go here. Scaling preferences exist in the general program preferences as well…. Read more »

Adding Statistics to Samples

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To add a statistic or statistics to samples in the workspace, open the statistic interface. Information on statistics can be found here.

Graphs in the layout editor

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To place plots into the layout editor, just drag and drop samples or populations from the workspace into the layout editor window. For more details, please go here.

Statistics in the Table Editor

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To add statistics to the Table Editor, just drag and drop them from the workspace into the table editor interface. This document explains the process in greater detail.

Version 10 History

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Please find below a version history of FlowJo vX (version 10). 10.0.8 (Released December, 2014) 10.0.7 (Released December, 2014) 10.0.6 (Released March 18th, 2013) 10.0.5 (Released December 6, 2012) Fixed: Boolean Not gates Opening the Matrix Editor Canceling a Derived Parameter Compensation opening in a saved ACS file Compensation and reagent names are now saved in… Read more »

Plates

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Preferences for the Layout Editor Plate Tool and the Plate Editor are described here. Shared Options These options are used to designate the keywords from .FCS files that are used to annotate plates in the plate editor, and which files are associated with a plate in the layout editor plate tool.  There are three default… Read more »

Legacy MoFlo and Legacy Cyan

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The legacy MoFlo and Cyan produce FCS2.0 files from Summit v4 or less which are analog (FCS2.0) files. Analog data (or FCS2.0) data files are read by FlowJo in the format they were exported.  Analog data files are exported with data scaling information and compensated fluorescence values (if compensation was done on the instrument).  Therefore, all FCS2.0 data… Read more »

FC500

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The FC500 produces a LMD file that contains a fcs2.0 portion and a fcs3.0 portion. Simply setting the preferences in v10 to read the fcs2.0 portion will ensure that the data is displayed in FlowJo as it was shown on the FC500.