Plate Editor Titration

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Use the plate editor to quickly make a titration (dilution factor) keyword for your samples The plate editor in v10 has a function to create a titration keyword and add a multiplier value to each sample in a 8 sample series. The keyword can be concentration like 1ug/ml, 0.5mg/ml, .25ug/ml, etc. The keyword can also… Read more »

Importing metadata from a CSV file

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Use Excel to add and organize your keywords and import that right into FlowJo to annotate your data files. Many users keep an excel spreadsheet of their samples and information about the sample to help organize their data files and keep track of them, particularly when using plates. With FlowJo v10’s new plate editor, you can actually save that… Read more »

BifurGate Tool

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Right-clicking on a file in the workspace or a sub-population includes an option for automatically creating bisector gates. First, why “BifurGate?” Bifurcate is a verb which means “to divide into two branches or forks,” and a gate is a selected subpopulation.  Putting these works together gets us BifurGate, which will divide populations into two gates… Read more »

Learn FlowJo Now!

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I downloaded FlowJo v10 now what?! Get started with some free tutorials, including demo data and learn how to do your analysis in just a few clicks.   The tutorial’s demo data contains a set of FCS raw data and a detailed printable instruction manual (PDF, DOC, or TXT file).  Don’t have time to read the manuals? No… Read more »

Pseudocolor Plots

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The ever-popular pseudocolor plot is back again in FlowJo v10! Extract more relevant information from your graphical display, get a “feel” for your data, and wow your co-workers with a colorful display of your experimental results. Pseudocolor plots are a type of bivariate density plot. That is, they display the relative population density of cell… Read more »

Drag-To Guides

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Guides are a simple way to align objects’ edges in the Layout Editor. Under the Edit Tab, in the Show & Hide Band, is a toggle button to Show Guides. To create new Guides, drag either ruler into the layout.  Objects that are dragged in the vicinity of a Guide will appear to snap to it… Read more »

Sidebar

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The right side of the Layout Editor contains a sidebar of extra options called Properties. There is a copy of the Layouts Band (Layouts tab), convenient for those who need more than a handful of layouts in the experiment and don’t want to go back to the Layout Editor Ribbon. There is a copy of… Read more »

Visualizing Plate-based Data

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The tool to assist in rapid visualization of plate-based flow data statistics is located in the Layout Editor. Open the Layout Editor and select the Plate tool. A click, drag and release movement with this tool in the layout editor will create a single open cell. Within the workspace, select the group you wish to… Read more »

Adding Keywords from a Spreadsheet

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Keywords, or columns of keywords, can be copied from a spreadsheet and pasted into a FlowJo Workspace. To add a spreadsheet list as keywords to the Workspace: From your spreadsheet program, copy the column of values you would like to add to the Workspace, click on the first cell in the Workspace and use Ctrl+V to… Read more »

Power Band

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The Power band contains the auxiliary tool Diagnostics to allow trouble shooting of the software Diagnostics What more frustrating that the software freezing on you while doing your analysis. This tool will help FlowJo support team to know the “why?” and fix it in our next update.