There are numerous ways to export figures for publication or presentation purposes. They are all explained in detail here.
Version 10 History
Please find below a version history of FlowJo vX (version 10). 10.0.8 (Released December, 2014) 10.0.7 (Released December, 2014) 10.0.6 (Released March 18th, 2013) 10.0.5 (Released December 6, 2012) Fixed: Boolean Not gates Opening the Matrix Editor Canceling a Derived Parameter Compensation opening in a saved ACS file Compensation and reagent names are now saved in… Read more »
Plates
Preferences for the Layout Editor Plate Tool and the Plate Editor are described here. Shared Options These options are used to designate the keywords from .FCS files that are used to annotate plates in the plate editor, and which files are associated with a plate in the layout editor plate tool. There are three default… Read more »
Legacy MoFlo and Legacy Cyan
The legacy MoFlo and Cyan produce FCS2.0 files from Summit v4 or less which are analog (FCS2.0) files. Analog data (or FCS2.0) data files are read by FlowJo in the format they were exported. Analog data files are exported with data scaling information and compensated fluorescence values (if compensation was done on the instrument). Therefore, all FCS2.0 data… Read more »
FC500
The FC500 produces a LMD file that contains a fcs2.0 portion and a fcs3.0 portion. Simply setting the preferences in v10 to read the fcs2.0 portion will ensure that the data is displayed in FlowJo as it was shown on the FC500.
CyAn ADP
CyAn ADP – Summit Software v5 Summit produces FCS 3.0 compliant file. FlowJo’s default preferences should display the data the same way as seen on the instrument. If you have any problems with the appearing differently in FlowJo then it did on the machine, please email techsupport [at] flowjo.com! It is a very easy fix!… Read more »
MoFlo XDP and Astrios
MoFlo XDP and MoFlo Astrios – Summit v6+ Summit produces FCS 3.0 compliant files that can be used with FlowJo. The data will look identical with the default settings, but the scale on the scatter parameters will be reported differently compared to the display on Summit. Summit appears to display data binned down to 256… Read more »
Gallios
Gallios currently uses CXP software Beckman-Coulter Gallios produces LMD files that contain an FCS 3.0 compliant portion. They display the data as collected, if “True View” is used. If standard view is used, linear parameters are scaled 1-1024 and log parameters to four decades with a range of 1-1024. This is a display similar to… Read more »
10.0.7 Release Notes
Below is a selection of our full list of new features and fixes in 10.0.7. For an exhaustive list, please see this page. Fixed: 10.0.7r2: Compensation is now “remembered” in templates Compensation population size restriction has been reduced to a warning if under 5%. Manual gating will override Compensation Matrix calculation and allow matrix to calculate… Read more »
Script Editor
The Script Editor is a platform that allows the user to program their own routines into FlowJo using JavaScript without having to worry about compiling a program. The script editor allows low-level access to FlowJo, and uses JavaScript to execute some powerful operations. The editor can be found by default in the Cytometry band… Read more »