The right side of the Layout Editor contains a sidebar of extra options called Properties. There is a copy of the Layouts Band (Layouts tab), convenient for those who need more than a handful of layouts in the experiment and don’t want to go back to the Layout Editor Ribbon. There is a copy of… Read more »

The tool to assist in rapid visualization of plate-based flow data statistics is located in the Layout Editor. Open the Layout Editor and select the Plate tool. A click, drag and release movement with this tool in the layout editor will create a single open cell. Within the workspace, select the group you wish to… Read more »

Keywords, or columns of keywords, can be copied from a spreadsheet and pasted into a FlowJo Workspace. To add a spreadsheet list as keywords to the Workspace: From your spreadsheet program, copy the column of values you would like to add to the Workspace, click on the first cell in the Workspace and use Ctrl+V to… Read more »

The Statistics band makes it easy to add statistical information about the selected sample to the Workspace. When you select a sample and use one of the functions on this band, FlowJo computes the desired statistic for you and displays it beneath the chosen sample in the workspace. Add Statistic Clicking this icon opens up… Read more »

This band provides the opportunity to create new gates, edit sample annotations, view metadata, and rename populations. Rename Clicking this button allows you to change the given name of the selected population. Compare Populations A platform for comparing the differences between two or more files using statistical models. The tools included allow the user to… Read more »

The Select band is located under the Edit tab of your Workspace. This band contains three icons that provide sample and population node selection options as described below. Select Samples Clicking Select Samples reveals a dropdown menu which allows you to select an alternating sequence of samples beginning with the currently selected sample. For example,… Read more »

The Settings band allows the user to customize his or her personal configuration of FlowJo. Here the user can adjust the width and content of columns displayed in the workspace, and show or hide statistics. Edit Columns Clicking this icon opens a dialog box that allows the user to add or remove keyword columns to… Read more »

Selecting an Expand/Collapse icon provides four actions to manipulate the display of a Workspace by expanding or collapsing the analysis hierarchy of a particular sample or a group of samples. These functions reduce scrolling when you are analyzing a large number of samples. Expand Selection Reveals any hidden items in the analysis hierarchy of the selected population… Read more »

There are two action icons located within the Find band that allow you to search for FCS data files or text within these data files. These actions can help you quickly locate and/or replace keywords and files from your Workspace. Find Search for text in keywords of the data files. FCS Scan Process your local… Read more »

From the FlowJo Navigate band, users can open FlowJo’s Table Editor and Layout Editor. This band also allows the user to create a new workspace, access preferences, add a new group to the existing workspace, or browse the file system for samples (in .FCS format) to add to the current workspace. New Workspace Clicking this icon will… Read more »