The right side of the Layout Editor contains a sidebar of extra options called Properties. There is a copy of the Layouts Band (Layouts tab), convenient for those who need more than a handful of layouts in the experiment and don’t want to go back to the Layout Editor Ribbon. There is a copy of… Read more »

The tool to assist in rapid visualization of plate-based flow data statistics is located in the Layout Editor. Open the Layout Editor and select the Plate tool. A click, drag and release movement with this tool in the layout editor will create a single open cell. Within the workspace, select the group you wish to… Read more »

Keywords, or columns of keywords, can be copied from a spreadsheet and pasted into a FlowJo Workspace. To add a spreadsheet list as keywords to the Workspace: From your spreadsheet program, copy the column of values you would like to add to the Workspace, click on the first cell in the Workspace and use Ctrl+V to… Read more »

This band provides the opportunity to create new gates, edit sample annotations, view metadata, and rename populations. Rename Clicking this button allows you to change the given name of the selected population. Compare Populations A platform for comparing the differences between two or more files using statistical models. The tools included allow the user to… Read more »

The Select band is located under the Edit tab of your Workspace. This band contains three icons that provide sample and population node selection options as described below. Select Samples Clicking Select Samples reveals a dropdown menu which allows you to select an alternating sequence of samples beginning with the currently selected sample. For example,… Read more »

The Settings band allows the user to customize his or her personal configuration of FlowJo. Here the user can adjust the width and content of columns displayed in the workspace, and show or hide statistics. Edit Columns Clicking this icon opens a dialog box that allows the user to add or remove keyword columns to… Read more »

The Statistics band makes it easy to add statistical information about the selected sample to the Workspace. When you select a sample and use one of the functions on this band, FlowJo computes the desired statistic for you and displays it beneath the chosen sample in the workspace. Add Statistic Clicking this icon opens up… Read more »

The Groups band contains functions that allow the user to create and manipulate groups from the samples in the workspace. From here the user can group selected samples together, create groups based on a certain keyword (i.e. a group of all samples with the same Patient ID), or copy specific values or gates to existing… Read more »

The Help band contains information about using FlowJo. From this band the user can check version info, access tutorials, user manuals, email flowjosupport@bd.com and link to the official FlowJo blog. Workspace Links Workspace links contains a set of links to this help documentation regarding the workspace. About Clicking the About icon opens a dropdown menu… Read more »

The Cytometry band allows users to access additional platforms required for specific analyses. Compensation Compensation will provide an interface to identify single stain controls, set proper gates and create the compensation matrix.  This process is fundamental to accurately analyzing multicolor immunofluorescence flow cytometry data. This button in the workspace allows access the compensation editor interface, where… Read more »