The Groups band contains functions that allow the user to create and manipulate groups from the samples in the workspace. From here the user can group selected samples together, create groups based on a certain keyword (i.e. a group of all samples with the same Patient ID), or copy specific values or gates to existing… Read more »

The Help band contains information about using FlowJo. From this band the user can check version info, access tutorials, user manuals, email flowjosupport@bd.com and link to the official FlowJo blog. Workspace Links About Clicking the About icon opens a dropdown menu with two options. The “About” button opens a window that contains version, engine, Java,… Read more »

The Cytometry band allows users to access additional platforms required for specific analyses. Compensation Compensation will provide an interface to identify single stain controls, set proper gates and create the compensation matrix.  This process is fundamental to accurately analyzing multicolor immunofluorescence flow cytometry data. This button in the workspace allows access the compensation editor interface, where… Read more »

Keywords used in the FlowJo workspace, are valuable annotation embedded in FCS data files. You can utilize them to sort and filter samples based on their annotation characteristics. These keywords are combined with groups to effectively subset analyses into components. Add Keyword Clicking this icon allows the user to create a new attribute/value pair for… Read more »

The Experiment band allows users to include additional information in an analysis Add Keyword Click on that button to add additional keyword-value pairs to the Workspace Annotate Experiment This dialog supports the new conventions for labeling metadata covered by MIFlowCyt. Plate Editor Access the Plate Editor. The Plate Editor can annotate FCS files from plate-based assays.

The Clipboard band contains functions that allow the user to duplicate or remove samples, statistics, or pieces of text from the Workspace. The most recently cut or copied item is saved to the clipboard and can be inserted back into the workspace using the Paste function. Cut The Clipboard band contains functions that allow the… Read more »

The Power band provides access to diagnostic and benchmarking functions. This should not be required for routine use of the program, but is helpful if attempting to resolve problematic cases.  Power Band Diagnostics Open windows with more information about the workings of the program. Extra Open FlowJo server window and other cool stuff.

The Document band allows the user to save changes to the workspace, revert to the last save state, or export the workspace to the user’s hard drive. It also contains functions for renaming and inspecting selected samples from the workspace and opening the graph windows for those samples.   Print Clicking this icon opens a… Read more »

FlowJo’s Biology Band contains a series of tools for applying mathematical or statistical models to flow data. Some of the tools are intended to be used with specific biological systems. Biology Band Cell Cycle Creates a one-dimensional model of DNA content for the selected population. To use this tool, click on a population at any… Read more »

The Boolean gates ribbon contains a series of tools for using existing gates to create additional, more complex gates. Boolean Band   Create Combination Gates Create Combination Gates makes a set of Boolean gates comprised of all possible combinations of the gates selected at the time this function is chosen. For example, if you have gates… Read more »