New Features: New tSNE Platform now offers super high performance dimensionality reduction. Warnings: For tSNE mappings in the latest release, parameters should not be scaled using log or lin transformations. We generally recommend using Biex transformations for those parameters. Log and lin transformed parameters can lead to over-clustering within the tSNE embedded space. Security protocols… Read more »

Updates New native tSNE platform. Note: This does not replace the legacy tSNE plugin (as that will be required as an object within FlowJo’s plugin folder) but is a preferable method of calculating derived parameters, due to its supremely improved performance. Derived parameter fixes. Memory management issues fixed. Native tSNE now runs correctly on grandchild… Read more »

New Features: New tSNE Platform now offers super high performance dimensionality reduction. Warnings: Security protocols that change SSL certificates will interfere with authentication of FlowJo via FlowJo Portal licensing method. Contact your local IT personnel and/or office@flowjo.com to troubleshoot issues with Portal authentication. Changes in scaling within FlowJo v10.5+ relative to previous versions can cause… Read more »

Updates New and more powerful computing engine – Data in this engine is built on top of the Eigen library, allowing us to store data more efficiently, and thereby giving data a smaller memory footprint and better performance in computations. Now offering named-user subscription (i.e. username/password) option for authentication through the FlowJo Portal platform. FlowJo is now fully… Read more »

New Features: FlowJo Portal Authentication is now available, enabling named-user subscription (i.e. username/password). Includes a new and enhanced computing engine, for improved performance. Drag and drop merging of CSV parameters into data matrices now supported. FlowJo is now fully GDPR compliant. Warnings: When saving an ACS file from a FlowJo Envoy based workspace, the default location prompt… Read more »

Within FlowJo v10.5+ new parameters saved in CSV format can be merged with existing data matrices by dragging and dropping directly onto appropriately matched data files. How To Mergeable CSV files must meet certain criteria in order to merge correctly with the target data file: The CSV to merge must have the same number of… Read more »

FlowJo v10.5+ offers a named-user subscription (i.e. username/password) option for authentication through the FlowJo Portal platform. This latest licensing tool means researchers no longer need to worry about losing or breaking their FlowJo Dongle, and their license won’t be bound to a particular computer in order to analyze data in FlowJo. For detailed information regarding the… Read more »

Updates User interface on HDPI displays now correctly sized at install time. Fixed incorrect statistics generated from certain MQD data files. Fixed issue of Downsample populations being dropped from ACS files. Corrected $PnE keyword value changes occurring during export. FACSLyric data now utilizes standard BD transformations by default. Known Issues Authentication 24-hour offline grace period… Read more »

New Features FlowJo icons on high resolution monitors now correctly sized. FACSLyric data now gets its own badge in FlowJo’s Cytometers preferences, and utilizes standard BD transformations by default. Bugs Fixed Fixed incorrect statistics reported for some MQD data files. Downsample populations no longer dropped from ACS files. $PnE keyword value no longer altered during export…. Read more »

The Spillover Spreading Matrix (SSM) is available from the Compensation Editor. This tool works for matrices calculated within FlowJo’s Compensation Wizard only, it isn’t possible to calculate SSM for acquisition defined compensation matrices calculated beforehand.   Calculating an SSM To generate a spillover spreading matrix in FlowJo visit the compensation editor, select the matrix of… Read more »