10.4.2 Release Notes

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New Features FlowJo icons on high resolution monitors now correctly sized. FACSLyric data now gets its own badge in FlowJo’s Cytometers preferences, and utilizes standard BD transformations by default. Bugs Fixed Fixed incorrect statistics reported for some MQD data files. Downsample populations no longer dropped from ACS files. $PnE keyword value no longer altered during export…. Read more »

Spillover Spreading Matrix

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The Spillover Spreading Matrix (SSM) is available from the Compensation Editor. This tool works for matrices calculated within FlowJo’s Compensation Wizard only, it isn’t possible to calculate SSM for acquisition defined compensation matrices calculated beforehand.   Calculating an SSM To generate a spillover spreading matrix in FlowJo visit the compensation editor, select the matrix of… Read more »

10.4.1 Exhaustive Release Notes

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Updates Search bar added to parameter selectors in the graph window. Color mapped (aka “heat map statistic”) parameter labeling in layouts. Stopped Compensation Wizard matrix re-calculations (ad infinitum). Workspace Title will no longer automatically revert to “unsaved” immediately after a save. Special considerations for Cytek data scaling implemented. Color Mapping axis keyboard shortcuts are now functional…. Read more »

10.4.1 Release Notes

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New Features Search bar added to parameter selectors in the graph window. Color mapped (aka “heat map statistic”) parameter labeling in layouts. Bugs Fixed Proliferation Platform Improvements Stopped Compensation Wizard matrix re-calculations (ad infinitum). More Info For more details on features in this release, and a comprehensive list of known issues visit the exhaustive release notes…. Read more »

Color Mapping

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Color mapping in FlowJo’s graph window allows users to visualize a third parameter in the two-dimensional display, by illustrating a statistical value for any tertiary parameter in a color scale applied to the dots displayed. Accessing the Color Map Checking the box “Color Axis” will display a third parameter by color within the graph window:… Read more »

10.4 Exhaustive Release Notes

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Below are the exhaustive release notes and known issues. For the general release notes, please see this page. Updates FlowJo Enterprise (deprecated) button now replaced with a FlowJo Envoy Integration Help button. Updated iconography throughout. Updated Java Runtime Environment – version 1.8.0_144. Known Issues Proliferation: This feature may fail to correctly model certain data files. This… Read more »

10.4 Release Notes

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New Features: Hotly anticipated color mapping within the graph window, to illustrate a third parameter there is now available. Spillover spreading matrix (SSM) now available in the Compensation Editor. Warnings: When saving an ACS file from a FlowJo Envoy based workspace, the default location prompt is a temporary cache folder, which will be deleted when FlowJo… Read more »

BD Research Cloud Integration

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BD Research Cloud is now integrated with FlowJo v10.8! BD Research Cloud is an application for communication and collaboration across a lab’s entire toolbox, including but not limited to flow cytometry and sequencing processes. Being integrated directly into the FlowJo workspace means you can access any analyses and data-sets shared with you in BD Research… Read more »

10.3 Release Notes

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New Features: We are happy to announce that the Proliferation Platform is here! We have revised the Logicle Transform implementation. Existing workspaces with gates using this transform may need to be adjusted. Warnings: Because FlowJo® now uses Java 8, we no longer support Mac OS 10.6.8 (because it does not support Java 8). Major Changes:… Read more »

10.3 Exhaustive Release Notes

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Below are the exhaustive release notes and known issues. For the general release notes, please see this page. Known Issues Compensation: A BD 25-color data file may not include the compensation matrix when exported from and brought back into FlowJo. Workaround: Export the compensation matrix as an MTX file, and re-apply it to the exported data… Read more »