10.4 Release Notes

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New Features: Hotly anticipated color mapping within the graph window, to illustrate a third parameter there is now available. Spillover spreading matrix (SSM) now available in the Compensation Editor. Warnings: When saving an ACS file from a FlowJo Envoy based workspace, the default location prompt is a temporary cache folder, which will be deleted when FlowJo… Read more »

10.3 Release Notes

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New Features: We are happy to announce that the Proliferation Platform is here! We have revised the Logicle Transform implementation. Existing workspaces with gates using this transform may need to be adjusted. Warnings: Because FlowJo® now uses Java 8, we no longer support Mac OS 10.6.8 (because it does not support Java 8). Major Changes:… Read more »

10.3 Exhaustive Release Notes

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Below are the exhaustive release notes and known issues. For the general release notes, please see this page. Known Issues Compensation: A BD 25-color data file may not include the compensation matrix when exported from and brought back into FlowJo. Workaround: Export the compensation matrix as an MTX file, and re-apply it to the exported data… Read more »

Discovery Example

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Discovery Example The CellOntology plugin is extremely useful for discovery work, especially when investigating new populations of cells that you may be unfamiliar with. Consider the following example: We have a multi-parametric data set with a panel that includes the standard T, B, and monocyte subsets. In addition to the standard panels, we have included markers that we are less familiar with but… Read more »

10.2 Release Notes

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New Features Note:  This version of FlowJo is compatible with FlowJo Enterprise v2.0 or the newly released FlowJo Enterprise 2.1. Be sure your version of FlowJo Enterprise is new enough before installing this version of FlowJo. New FlowJo Plugin Architecture.   FlowJo can now use plugins that can perform a variety of functions, including the creation… Read more »

FlowMeans

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FlowMeans is method for automated identification of cell populations in flow cytometry data based on K-means clustering. The FlowMeans algorithm utilizes the R statistical computing environment and is implemented in FlowJo 10.1r7 as a plugin accessible from the Plugins menu (Workspace tab –> Populations band). Setup If you have never used a FlowJo Plugin, please see… Read more »

FlowJo 10.1r7 Exaustive Release Notes

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Below are the exhaustive release notes and known issues.  For a digest of these release notes, please see this page. Bugs Fixed Improved the file saving behavior with ACS files Fixed an issue where very large and complex workspaces could take a very long time to close. (A side effect of this is that you… Read more »

10.1r7 Release Notes

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New Features Note:  This version of FlowJo is NOT compatible with FlowJo Enterprise. Stay tuned for the upcoming FlowJo Enterprise release that will work with this version. New FlowJo Plugin Architecture.   FlowJo can now use plugins that can perform a variety of functions, including the creation of populations, creation of derived parameters, and actions when… Read more »

FlowJo Enterprise Band

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The FlowJo Enterprise band contains functions for connecting to your FlowJo Enterprise server, uploading protocols and compensation matrices, and distributing plugins.   The FlowJo Enterprise task, when clicked, will connect you to the FlowJo Enterprise server web page. This URL (web address) is unique to your site, and you must be an Enterprise subscriber to access it…. Read more »

DownSample

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Downsampling is the process of selecting a limited number of data points/events from a sample or gated population. A new Downsample Gate is created containing the selected subset of events, which can be used like any other gated subset or population node within FlowJo. Downsampling is often a necessary prerequisite to algorithmic calculations like t-Distributed Stochastic Neighbor Embedding (tSNE), as… Read more »