10.5 Release Notes

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New Features: FlowJo Portal Authentication is now available, enabling named-user subscription (i.e. username/password). Includes a new and enhanced computing engine, for improved performance. Drag and drop merging of CSV parameters into data matrices now supported. FlowJo is now fully GDPR compliant. Warnings: When saving an ACS file from a FlowJo Envoy based workspace, the default location prompt… Read more »

CSV Parameter Merging

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Within FlowJo v10.5+ new parameters saved in CSV format can be merged with existing data matrices by dragging and dropping directly onto appropriately matched data files.   How To Mergeable CSV files must meet certain criteria in order to merge correctly with the target data file: The CSV to merge must have the same number… Read more »

FlowJo Portal Licensing

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FlowJo v10.5+ offers a named-user subscription (i.e. username/password) option for authentication through the FlowJo Portal platform. This latest licensing tool means researchers no longer need to worry about losing or breaking their FlowJo Dongle, and their license won’t be bound to a particular computer in order to analyze data in FlowJo. For detailed information regarding the… Read more »

10.4.2 Exhaustive Release Notes

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Updates User interface on HDPI displays now correctly sized at install time. Fixed incorrect statistics generated from certain MQD data files. Fixed issue of Downsample populations being dropped from ACS files. Corrected $PnE keyword value changes occurring during export. FACSLyric data now utilizes standard BD transformations by default. Known Issues Authentication 24-hour offline grace period… Read more »

10.4.2 Release Notes

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New Features FlowJo icons on high resolution monitors now correctly sized. FACSLyric data now gets its own badge in FlowJo’s Cytometers preferences, and utilizes standard BD transformations by default. Bugs Fixed Fixed incorrect statistics reported for some MQD data files. Downsample populations no longer dropped from ACS files. $PnE keyword value no longer altered during export…. Read more »

Spillover Spreading Matrix

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The Spillover Spreading Matrix (SSM) is available from the Compensation Editor. This tool works for matrices calculated within FlowJo’s Compensation Wizard only, it isn’t possible to calculate SSM for acquisition defined compensation matrices calculated beforehand.   Calculating an SSM To generate a spillover spreading matrix in FlowJo visit the compensation editor, select the matrix of… Read more »

10.4.1 Exhaustive Release Notes

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Updates Search bar added to parameter selectors in the graph window. Color mapped (aka “heat map statistic”) parameter labeling in layouts. Stopped Compensation Wizard matrix re-calculations (ad infinitum). Workspace Title will no longer automatically revert to “unsaved” immediately after a save. Special considerations for Cytek data scaling implemented. Color Mapping axis keyboard shortcuts are now functional…. Read more »

10.4.1 Release Notes

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New Features Search bar added to parameter selectors in the graph window. Color mapped (aka “heat map statistic”) parameter labeling in layouts. Bugs Fixed Proliferation Platform Improvements Stopped Compensation Wizard matrix re-calculations (ad infinitum). More Info For more details on features in this release, and a comprehensive list of known issues visit the exhaustive release notes…. Read more »

Color Mapping

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Color mapping in FlowJo’s graph window allows users to visualize a third parameter in the two-dimensional display, by illustrating a statistical value for any tertiary parameter in a color scale applied to the dots displayed. Accessing the Color Map Checking the box “Color Axis” will display a third parameter by color within the graph window:… Read more »

10.4 Exhaustive Release Notes

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Below are the exhaustive release notes and known issues. For the general release notes, please see this page. Updates FlowJo Enterprise (deprecated) button now replaced with a FlowJo Envoy Integration Help button. Updated iconography throughout. Updated Java Runtime Environment – version 1.8.0_144. Known Issues Proliferation: This feature may fail to correctly model certain data files. This… Read more »