Illustrating the expression of many genes within a plot of interest was a tedious process, until now!

Multigraph color mapping is a feature in SeqGeq, which illustrates many copies of a chosen plot from the Layout Editor, and color maps each by a different gene selected. This is particularly useful for exploring different aspects of dimensionally reduced plots, such as tSNE.

How To

To implement a multigraph color map within the Layout Editor, simply right click on a plot there, mouse over the “Make Multigraph Overlays” option there, and select “Multigraph Color Mapping”:

This will prompt you with a parameter selection dialog where you can select the parameters you’d like to iterate over within the color mapping:

Examples

Multigraph color mapping allows users to very quickly and efficiently explore categorical parameters of interest. In the following case we have labeled cells by tissue type (based on index sorting in flow cytometry), and disease state versus control, and store this information in a categorical parameters:

In multi-omic data a set of parameters from one domain can be color mapped onto the results from another. In the following image we illustrate parameters from index sorting in flow cytometry directly onto a tSNE mapping derived from single-cell RNA sequencing parameters:

If you have any questions about exploring your data in SeqGeq or the Multigraph Color Mapping feature in particular, please reach out: seqgeq@flowjo.com