Updates

  • Embed: Forward propagate clustering or dimensionality reduction results to new files with the same parameters.
  • Autofluorescence extraction in traditional (gate-based) compensation: Use an unstained cell data file and true zero as the background value to model autofluorescence for extraction.
  • MEM – Marker Enrichment Modeling (MEM): Re-name clusters automatically and quantitatively based on their marker expression with a human- and machine-readable label.
  • Spectral similarity scores: Calculate the cosine index for acquired data to evaluate reagent choices made in panel design based on data from your specific instrument and experimental conditions.
  • Auto-Quad gates: Automatically create quads and rectangle gates based on range gates, bi-sector gates or the data itself.
  • Clone gates: Gates with the same name can now be linked together as ‘clones’ so that their position is synchronized.
  • Native Cluster Explorer: Display line charts, bar charts, heat maps, and dimensionality reduction plots of clustered data in FlowJo without the use of a plugin.
  • Improved interactions with BD® Research Cloud: Save and load options throughout the software now include the BD® Research Cloud as an option.
  • There are now caching preferences in FlowJo for handling file downloading from the BD® Research Cloud.
  • Derived parameter scale changes preserved through save/re-open a workspace
  • Graph windows open offscreen on Windows bug fixed
  • Long network drive paths fixed. Save locations on cloud or network based drives can have longer file paths than allowed by a local computers operating system. We have updated our open/save menus to display these longer paths.
  • Attempting to save to a folder that has been deleted will now produce an error message.
  • Box Drive will be presented as a save/open option.
  • Double click on a saved workspace to with FlowJo running will now open a second workspace window.
  • The time parameter is scaled using the $TIMESTEP keyword, such that its units are in seconds.  If the $TIMESTEP keyword is not present, FlowJo 10.9 will use $BTIM, $ETIM, and the first and last recorded event times to determine a rescale factor. Previous versions of FlowJo performed that operation in the opposite order, making $BTIM /$ETIM the primary ‘truth’.
  • The table editor can include samples with the same name in a batch. Previously, two samples that had the same name, i.e. two plates loaded into a workspace would produce identically named A01 files, would be treated as duplicates during batching from the table editor and one would be omitted from the output.
  • The color palette legend has been expanded to allow 24 colors to be pre-defined in a specific order, allowing for finer control of overlays within the layout editor.
  • The color palette legend order is now followed exactly. Previously colors 0 and 1 would flip on add of the second color.
  • The number of decimal places displayed in the SSM / TSM has been trimmed to two for easier readability.
  • The default preferences for Cytek Aurora data files have been updated to reflect the current cytometer defaults.
  • Default preferences have been added for Cellular Highway machines.
  • The bug when using Portuguese to use $ as a decimal place holder has been fixed.
  • Multigraph plots now show the name of the sample when batching.
  • the default suggested name when adding a keyword has been changed to SpecimenID, away from SampleID which is a commonly used keyword.
  • Header and Footer preferences now apply without a restart.
  • LMD files exported from the Navios display the parameter names in the correct order. Previously there was re-ordering.
  • LMD files exported from the Astrios now display all parameters. Previously some had been hidden.
  • Plugin nodes will not recalculate on transform updates.
  • 100th percentile statistic now viable. Previously the 100th percentile would not calculate.
  • Bug preventing quad gate stats from updating the displayed value when an offset was introduced is fixed.

Known Issues

  • For any statistics created on a mathematically derived parameter, the stat will not update beyond one change to the derived parameter equation.
  • Gallios LMD files do not reflect LMD defaults. T-button scaling does work properly to allow the user to use whatever scale they chose.
  • ACS files will not save if a # is included in the name.
  • The Moom and Cylance applications interfere with FlowJo performance.
  • Font size in text boxes in the layout editor is non-responsive after duplicate or copy-paste actions.
  • In the native version of cluster explorer, populations with less than 0.5% of the events are rendered to small to see in the bar charts, and bar charts do not update when subset nodes are selected.
  • Additionally, the mouseover functionality in native cluster explorer displays the data of the column to the left as opposed to the column directly moused over.
  • The parameter selector in the Color by Expression Level tool of the native cluster explorer and the displayed parameter are misaligned.
  • When doing spectral compensation using AutoSpill, optimize weights does not work.