To install FlowJo, please download the installer from our website at:

You can download the installer appropriate for your platform (Mac or PC), by visiting the Download tab there.

  • For a PC, download the installer.exe file and open it to install the program.  Choose to place a shortcut on the desktop, and when the installer finishes double-click on the FlowJo shortcut icon to launch the program.
  • For a Mac, download the installer.dmg file and double-click to extract the program.  Once the DMG file is open, drag the application and plugins folder to your Applications folder, and double-click on the icon to launch FlowJo.

We also have more information about installing from the Website and navigating the license screens.

If you have downloaded the full version of FlowJo, until a serial number is provided, FlowJo will only load specially enabled demonstration data files. To check license status of FlowJo, please visit the License section of FlowJo’s preferences. To obtain a trial 30-day serial number so that you can try FlowJo using your own data simply fill out the trial license form.

The Windows installer is not a certified software, nor is the Mac version available from AppStore. This may raise security flags, which IT is usually best equipped to troubleshoot.



Java – SE v1.8 is current, as of 28 March, 2018.

Microsoft Visual C++ Redistributable Package, 2010.

The “.net” (dot-net) 2 driver (or greater).


Other Resources:

Tips for Site Wide Installations

Common Install Issues

Silent Install Instructions – The command script to run a silent installation is: “EXECUTABLE_NAME -i silent“. Where an example EXECUTABLE_NAME is “FlowJo-Win64-10.4.2”, for the 64bit Windows installer of FlowJo v10.4.2.


For any issues installing FlowJo, please reach out to tech support: