Select the Layouts button in the Preferences window to open the Layouts-specific preferences.

Layout Options
Batch Format: This specifies where a new batch will be output; applies to new layouts in new workspaces. A batch report can be exported to a new layout, or to several other export options.
Default Zoom: Choose the default zoom for newly opened layouts.
Iteration Type: Select Sample from this menu to prepare the Layout Editor for batching. More
batching options are described here. The Off setting prepares a static layout.
Destination Folder: Choose a destination folder for layout output files.
Plot Orientation Horizontal: If checked, a selection of multiple populations dragged to the layout will be oriented horizontally.
Use Placeholders: Select this box to use empty placeholder plots instead of visible plots in the layout. This speeds up work on large, complex analyses.
Batch Row Width: Set the width of the Batch Row.
Double-Click opens Graph: This toggle governs whether double-clicking opens a graph window or chart properties.
Offset Histogram Overlays: Select this box to offset overlaid histograms vertically by default.
Fill Histogram Overlays: Solid colored fields under the distribution.
Drag Mismatch Policy: If batching is set to a sample name, and a different sample is dragged in to the Layout Editor, this setting tells FlowJo to:
- Turn Iteration off
- Change the dragged sample to the one currently specified in the Layout.
- Change the Layout setting to match the new sample.
- Cancel the action.
- Ask the user to choose.
Extras:
Default Plate/Grid Size: Enter column value for Plate/Grid size.
Default Plate/Grid Size: Enter row value for Plate/Grid size.
Draw Borders Around Text: When checked, new text boxes have border by default.
Include Full Statistic On Drop: Add dropped statistics with name and parameter information.
Add Header: When checked, a default page header with time and document information is included.
Show Page Breaks: When checked, page boundaries and page numbers are drawn by default.
Add Footer: When checked, a default page footer with user and version information is included.
Show Background Grid: When checked, the background grid is visible by default.
Line Color: Set the line color for plates and grids of items in reports.
Minimal Chart In Grids: Hide axes and annotations for populations dragged into a grid.
Fill Color: Set the background color for plates and grids of items in reports.
Background Grid Size: Set the background grid size.