Graphs button in the Preferences tool to open the Graphs preferences. Descriptions are broken into sections below.

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Graph Type
Graph Type : Set the default graph type for all newly created graphs.

Contour Levels : Set the percentage of cells that each contour line represents, or switch to logarithmic scaling of the contour lines.

Smooth : If checked the spaces between data points will be filled with a gradual transition, generally using color.

Foreground Color : Set the color of the lines and dots used in drawing graphs.

Show Outliers : If checked, events outside of a smoothed representation of the data will be plotted as dots.

Background Color : Set the color of the filled space underneath graphs.

Histogram and Overlay Settings
Overlay Mode: Allows the user to select what type of y axis scaling will be used as the default histogram scaling when histograms are overlayed.

Line: Controls the default histogram line thickness.

Fill: Controls the default tint inside the histogram.

Options
Show Forward Scatter on X Axis : Will display forward scatter on X axis on all new plots.

Show Event Number Parameter : Add a parameter showing the events’ position within the data file

Show Uncompensated Parameters : If the file in compensated, define whether parameter menus include both compensated and uncompensated versions of each parameter.

Show Background Grid : If checked the graph will display lines extending the major ticks,

Number of dots drawn (0=all) : Select the MAXIMUM number of dots to display in a dot plot. Entering a 0 displays all events. Entering a number between 1 and 100 displays that percentage of events.

Show Warning if too many events on Axis :  If the number of events on either axis exceeds the specified threshold, FlowJo will display warning text.

Draw Large Dots : If checked, FlowJo will draw slightly bigger dots on dot plots.