Refer to the following external documents for more information regarding the Proliferation platform. Interpretation of Cellular Proliferation Data: Avoid the Panglossian. Mario Roederer, Cytometry Part A � 79A: 95 101, 2011 Assessing Antigen-Specific Proliferation and Cytokine Responses Using Flow Cytometry. Allsopp and Langhorne, Edited by Denise Doolan, Malaria Methods and Protocol, p. 409 Antigen/Mitogen-Stimulated Lymphocyte Proliferation. Whiteside, Measuring… Read more »

FlowJo has several analysis platforms that provide capabilities beyond the gating and statistics used for most simple data analysis.   Workspaces and Demo Data are provided for several of FlowJo’s platforms so that you can try out these features yourself . In addition, we have several short Tech Notes that give step-by-step instructions for FlowJo platforms… Read more »

To obtain a better fitting model, you can fix the location of the undivided population. Click on the curve of the undivided population and drag the center line to place the curve in the optimum position.  The black control handles can be dragged to independently adjust width and height, left and right arrow keys move… Read more »

Question 1: I have a CFSE plot where the majority of the cells are in the peak corresponding to four rounds of division. Yet- the proliferation index is only 2.14.  Although I understand that this is the relevant number and is what people use in the literature, I can’t explain why the proliferation index number is… Read more »

This page provides details on the Plate Editor tool. The Plate Editor has a ribbon with four tabs containing bands of available actions. The Plate Editor tab contains tools to create new, duplicate or delete plates, gather data from the Workspace and annotate: Annotate Experiment allows you to provide a description of your experiment following the MIFlowCyt standard. Read Samples from Group… Read more »

Tips for how to perform certain kinetics analyses. This list is not comprehensive; you should read the main Kinetics help page to understand how the platform works before you examine these tips. How can I determine the fraction of responding cells? The fraction of responding cells is defined as the percentage of events with a fluorescence… Read more »

Kinetics models output a set of summary statistics for each time range. On each kinetics model the statistics disclosure arrow can be opened to reveal a set of summary statistics.  Simply click the arrow on the right side of the model, as shown below, to view them.   The utility of the statistics will vary… Read more »

A threshold can be used to statistically define which cells are of interest Some of the statistics that can be selected from the kinetics Options box take a threshold as a parameter. These compute statistics on the events with a Y parameter value greater than the given threshold. Clicking on the “Set Threshold” button, or selecting… Read more »

Listed below is some external documentation regarding the Kinetics platform.   Intracellular ionized calcium, magnesium, membrane potential and pH. Rabinovitch and June, Flow Cytometry, by M. G. Ormerod Measurement of Calcium Mobilization Responses in Killer Cell/Target Conjugates by FACS Analysis. Vallitutti and Dessing, Natural Killer Cell Protocols, by Kerry S. Campbell, Marco Colonna Intracellular Calcium, Chapter 10… Read more »

This page is under construction; stay tuned for FlowJo Version 10.0.8 with Kinetics movies. This time series is from a calcium-flux experiment. In this experiment, peripheral T cells were stained with anti-CD markers to identify subsets and loaded with Indo-1. The ratio of Indo-1 for a gated subset of CD4 T cells is shown as… Read more »