Question 1: I have a CFSE plot where the majority of the cells are in the peak corresponding to four rounds of division. Yet- the proliferation index is only 2.14.  Although I understand that this is the relevant number and is what people use in the literature, I can’t explain why the proliferation index number is… Read more »

This page provides details on the Plate Editor tool. The Plate Editor has a ribbon with four tabs containing bands of available actions. The Plate Editor tab contains tools to create new, duplicate or delete plates, gather data from the Workspace and annotate: Annotate Experiment allows you to provide a description of your experiment following the MIFlowCyt standard. Read Samples from Group… Read more »

Tips for how to perform certain kinetics analyses. This list is not comprehensive; you should read the main Kinetics help page to understand how the platform works before you examine these tips. How can I determine the fraction of responding cells? The fraction of responding cells is defined as the percentage of events with a fluorescence… Read more »

Kinetics models output a set of summary statistics for each time range. On each kinetics model the statistics disclosure arrow can be opened to reveal a set of summary statistics.  Simply click the arrow on the right side of the model, as shown below, to view them.   The utility of the statistics will vary… Read more »

A threshold can be used to statistically define which cells are of interest Some of the statistics that can be selected from the kinetics Options box take a threshold as a parameter. These compute statistics on the events with a Y parameter value greater than the given threshold. Clicking on the “Set Threshold” button, or selecting… Read more »

Listed below is some external documentation regarding the Kinetics platform.   Intracellular ionized calcium, magnesium, membrane potential and pH. Rabinovitch and June, Flow Cytometry, by M. G. Ormerod Measurement of Calcium Mobilization Responses in Killer Cell/Target Conjugates by FACS Analysis. Vallitutti and Dessing, Natural Killer Cell Protocols, by Kerry S. Campbell, Marco Colonna Intracellular Calcium, Chapter 10… Read more »

This page is under construction; stay tuned for FlowJo Version 10.0.8 with Kinetics movies. This time series is from a calcium-flux experiment. In this experiment, peripheral T cells were stained with anti-CD markers to identify subsets and loaded with Indo-1. The ratio of Indo-1 for a gated subset of CD4 T cells is shown as… Read more »

The graph specifications control what metric is used on the Y-axis, and whether smoothing will be applied. When you click the Options disclosure triangle in the kinetics window, the options shown below appear. They modify the attributes of the current graph (or currently-selected gate.)   The ‘Statistic’ selection is the statistics that will be applied to… Read more »

FCS Scan provides an interface for locating FCS files from local or remote file systems and generating Workspaces from files located.  In situations where your IT department moves whole directories, or you can’t remember where you stored a sample set it can really come in handy. This tool is also extremely useful for finding files… Read more »

Data can be exported from the kinetics platform to the layout editor, the table editor, or as binned data. Exporting graphics to the layout editor Like any other node in FlowJo, the kinetics node can be dragged to the layout editor to create a graphic.  As you can see in the graphic below, the model… Read more »