The univariate cell cycle platform produces a number of statistics that can be used to summarize the modeling results.  This page is under construction; stay tuned for FlowJo Version 10 with Cell Cycle. FlowJo univariate Cell Cycle analysis can produce an array of statistics, which are shown in the upper portion of the model window, as… Read more »

This page contains suggestions for improving the fit of difficult to model cytometric data. Preprocessing Removing doublets, dead cells, and debris will improve the condition of data. Whatever steps you typically follow to do this can be applied to cell cycle data.  Some common methods for doing this are: Doublets can be removed by plotting… Read more »

There are four controllers for the 3D Viewer: Plot, Top Bar Controls, Parameters and Color   Plot From the Plot, set the parameters for X, Y and Z axes and assign a color if wanted. Setting Size: event size will scale based on how positive/negative events are for a specified parameter. More positive events will… Read more »

FlowJo is Copyright © Trustees of Leland Stanford Jr. University, 1996-1997 FlowJo is Copyright © FlowJo LLC., 1997-2019 FlowJo Tutorial, Tech Notes and Web Site are Copyright © FlowJo, LLC. 1997-2019 We are indebted to the Herzenberg Laboratory at Stanford and our tireless beta testers for comments and suggestions. Apache Batik technology for SVG is… Read more »

Welcome to FlowJo, a software application with an integrated environment for viewing and analyzing flow cytometric data. The environment is presented as the Workspace, which contains a list of loaded samples (experimental data), statistics, gates, and other analyses, as well as tabular and graphical layouts. The workspace is saved as a FlowJo document on your hard… Read more »