FlowJo’s backgating analysis provides a tool to view the effect of every gate in the gating tree of a multi-generation gated population. FlowJo’s backgating option generates graphs within which each level in the gating hierarchy is displayed, with the final gated population overlaid at each level. This overlay is a backgating overlay: in other words, it… Read more »

Add Annotations to your graphical reports in FlowJo to save time avoiding time-consuming searches through notebooks for experimental details. An Annotation automatically appears when plots are a dropped into the Layout Editor. The default annotation includes: Sample Name Population Name Population Event Count Adding annotations to graphical reports is useful for recalling certain aspects of a report and… Read more »

When you have more than one object selected in the Layout Editor, you can align them relative to each other. Allowing FlowJo to align your layout objects saves time and ensures accurate distribution and alignment. Choosing Arrange from the menu, FlowJo will display a list of alignment features. Here you can choose exactly how to align… Read more »

Adjunct Histograms display the univariate plots that correspond to the parameters used to create a bivariate plot. They can be a helpful tool, especially for visualizing edge events that may blend into the axes of a two dimensional plot. They are an available option in the Layout Editor. When displayed, the upper univariate plot (histogram) displays… Read more »

FlowJo is an integrated environment for viewing and analyzing flow cytometric data. FlowJo’s strength is in analyzing whole experiments encompassing many related samples. In addition to gating and statistics, FlowJo has specialized analysis platforms for assays involving DNA/Cell Cycle, Kinetics, and Proliferation, among others. FlowJo’s sophisticated tools allow for generation of statistics, graphs, tables, web… Read more »

FlowJo has several different output capabilities, encompassing graphics, statistics, and raw data. FlowJo supports printing from all the different views of data, as well as copying data through the clipboard or file system. FlowJo contains a special editor for the creation of Tables (statistical outputs) and Layouts (graphical outputs). The following is information regarding different aspects of… Read more »

Templates contain your entire analysis, minus the FCS files. They are the most efficient tool for repeated analyses.  A template is a replica of your workspace file without data.  It preserves groups, gates, table definitions, and layouts and allows all of that to be applied to the next data set.  A template can be created… Read more »

FlowJo  gives you the most flexibility possible with exported graphics. You can easily ungroup any graphic, and change the font attributes, line styles, etc., to make any graph look exactly the way you want in the Layout Editor. Hints on exporting graphs In the layout editor, you can copy/paste graphs to clipboard (copy/paste) to file (save… Read more »

The FlowJo workspace can be customized for frequently used operations using ribbons. The basic anatomy of ribbons are the tabs, and within each tab are multiple bands.  Figure 1 shows the FlowJo Workspace with its ribbon that organizes the features into a series of tabs, which facilitate easy navigation. Figure 1. The FlowJo Workspace Ribbon… Read more »

The contents of any window in FlowJo can be printed. To print the FlowJo Workspace window, select the Print command from the Document band. The Document band, shown in Figure 1, is by default located in the File ribbon. Alternatively, the print command can also be located in the FlowJo Home menu, shown in Figure 2. The FlowJo Home menu can be… Read more »