From the Layout Editor, graphical layouts can be exported in a variety of formats: – to clipboard (copy/paste) – to file (save or batch-to-file) Related Links: Batch Outputs Copy/Paste of images within FlowJo or to a 3rd party application like PPT (PowerPoint) If taking your data to PowerPoint or Microsoft Office products, the EMF format option… Read more »

Any text box within the FlowJo’s layout editor is capable of evaluating dynamic equations. These may be simple sums or ratios of statistics, or can be arbitrarily complex algebra formulas. To create a text box, simply click on the text tool in the toolbar. You can also just drag a statistic in to a layout… Read more »

In many ways, the Layout Editor is a like a simple page layout program. It can draw boxes and lines, type text and move the items around with a familiar interface. It has some additional aspects as well: it supports multiple pages each with its own layout defined; it has controls to apply the same layout… Read more »

FlowJo vX makes it easy to batch overlays and customize your graphical reports. Batching overlays is a powerful tool that enables you to view any number of control samples overlaid with test samples across an entire experiment. The general process is the same for all overlay batches: create an overlay in a layout, specify (lock)… Read more »

Although printing will remain the dominant way to summarize analyses for some time to come, there are times when being able to write direct to web pages can make it easier to share, publish or archive your experimental results. At the very least, this gives you the opportunity to check a lot of data, and… Read more »

FlowJo’s backgating analysis provides a tool to view the effect of every gate in the gating tree of a multi-generation gated population. FlowJo’s backgating option generates graphs within which each level in the gating hierarchy is displayed, with the final gated population overlaid at each level. This overlay is a backgating overlay: in other words, it… Read more »

Add Annotations to your graphical reports in FlowJo to save time avoiding time-consuming searches through notebooks for experimental details. An Annotation automatically appears when plots are a dropped into the Layout Editor. The default annotation includes: Sample Name Population Name Population Event Count Adding annotations to graphical reports is useful for recalling certain aspects of a report and… Read more »

When you have more than one object selected in the Layout Editor, you can align them relative to each other. Allowing FlowJo to align your layout objects saves time and ensures accurate distribution and alignment. Choosing Arrange from the menu, FlowJo will display a list of alignment features. Here you can choose exactly how to align… Read more »

Adjunct Histograms display the univariate plots that correspond to the parameters used to create a bivariate plot. They can be a helpful tool, especially for visualizing edge events that may blend into the axes of a two dimensional plot. They are an available option in the Layout Editor. When displayed, the upper univariate plot (histogram) displays… Read more »

FlowJo is an integrated environment for viewing and analyzing flow cytometric data. FlowJo’s strength is in analyzing whole experiments encompassing many related samples. In addition to gating and statistics, FlowJo has specialized analysis platforms for assays involving DNA/Cell Cycle, Kinetics, and Proliferation, among others. FlowJo’s sophisticated tools allow for generation of statistics, graphs, tables, web… Read more »