Compare a control to an experimental set, evaluate the accuracy of gates, find high or low expressors, or a perform a myriad of other analyses using Overlays in FlowJo v10. Overlaying univariate or bivariate plots is a powerful visual tool to for cytometric analyses. Differences between populations within a single experiment or amongst groups of experiments are… Read more »

If you select two or more objects in the layout editor, and then choose  (preferences)    from the Layouts menu you will see a rather imposing dialog with the title Set Attributes of Selected Items. This dialog is the union of all of the attribute editing dialogs that are available for editing arrows, boxes, graphs… Read more »

Make a Hollywood production of your flow cytometry data! If you want to track fluorescence over time, maybe upon activation or over a time series, a batched report as a movie can be incredibly useful! However, there are many uses for movies from reports in the Layout Editor. The movies generated in the Layout Editor are… Read more »

The Multigraph feature is a tool for exploring data in n-dimensions using a variety of graphical options. To access the Multigraph interface in version 10, place a plot in the layout editor and right click on the plot.  At the bottom of the right-click interface, there is an option for Multigraph called “Make Multigraph Overlay”. … Read more »

Layout Editor Ribbon   Layout Editor Tab   Layouts adds, copies, or deletes layouts Iteration groups samples included in the layout batches. For more on Iteration, click here. Batch generates a batch. For more on Batching, click here. File Tab   Document Band Print  opens the Print dialog, for more on printing, click here. Edit Header / Footer opens… Read more »

There are 3 lock features in the Layout Editor located on the Objects tab.   Any object, graph, statistic table, or graphic can use the Lock feature. Lock Position: Locks the position of the object. Lock Content: Locks statistics so iteration does not affect values. Locks content in text boxes and any item/object so changes cannot be made… Read more »

The Line/Arrow tool is used for drawing simple lines and arrows in the Layout Editor. Select this tool then click and drag anywhere in your layout to draw a line. To resize it, click on one of the square handles at the ends of the line. To move the line, move the mouse over the line… Read more »

Sample Mismatch!?! Now what? FlowJo can organize a batch by sample, by stain panel, or by keyword. (Complex batches can be organized by a sequence of these parameters.) Set these preferences with the drop-down menus at the top of the layout editor. If batching is set to a particular sample name, and a different sample is… Read more »

SAVE HOURS OF YOUR LIFE – BATCH! One of the strengths of computers is how they handle repetitive tasks. Show the computer how you want something done once, and it can repeat the task a million times without complaint. Flow cytometry often requires application of the same analysis to any number of samples, and FlowJo’s ability… Read more »

The Layout Editor’s Grid Tool makes it easy to organize your multi-plot layouts by grouping them into a single table. You can use grids for keeping graphs aligned and grouped, or you can model 96 well plates by dropping an entire plate of files onto the first cell of the grid. To create a grid:… Read more »