FlowJo’s  sophisticated Layout Editor  generates graphics that are publication-ready (or presentation-ready, for slides). The Layout Editor has a couple of different options for generating publication quality graphics (images). All graphics created in the Layout Editor (graphs, tables, text boxes, heatmaps, objects) can be exported immediately for publication or to other programs for further manipulation. For creating… Read more »

Creating PowerPoint Slides and working with images from FlowJo in Powerpoint There are several options for getting your analysis results and charts from the layout editor into Powerpoint, which are detailed below.  It is worth noting, however, that we have included many graphic and drawing tools within the Layout Editor so that you may manipulate graphics… Read more »

The Layout Editor in version 10 now allows you to create tables of statistics for a single sample’s entire hierarchy.  How is this different from previous versions?  Previous versions of FlowJo only allowed you to create tables with each sample as a row and the statistics, no matter at what population level in the hierarchy, always… Read more »

Placeholders are a way of letting FlowJo work with graphs inside a layout without the computation-intensive task of recalculating all the image information whenever you update a graph, gate or text box in the Layout Editor. The time to calculate a graph of a large data set can be several seconds. In a layout, which… Read more »

Resize the page, not the plots! A lot of time can be saved by understanding how to utilize page sizing in the Layout Editor. It is tempting to drag in a plot and adjust its size in order to get more plots on one page, but this is tiring and repetitive. FlowJo makes it easy… Read more »

Compare a control to an experimental set, evaluate the accuracy of gates, find high or low expressors, or a perform a myriad of other analyses using Overlays in FlowJo v10. Overlaying univariate or bivariate plots is a powerful visual tool to for cytometric analyses. Differences between populations within a single experiment or amongst groups of experiments are… Read more »

If you select two or more objects in the layout editor, and then choose  (preferences)    from the Layouts menu you will see a rather imposing dialog with the title Set Attributes of Selected Items. This dialog is the union of all of the attribute editing dialogs that are available for editing arrows, boxes, graphs… Read more »

Make a Hollywood production of your flow cytometry data! If you want to track fluorescence over time, maybe upon activation or over a time series, a batched report as a movie can be incredibly useful! However, there are many uses for movies from reports in the Layout Editor. The movies generated in the Layout Editor are… Read more »

The Multigraph feature is a tool for exploring data in n-dimensions using a variety of graphical options. To access the Multigraph interface in version 10, place a plot in the layout editor and right click on the plot.  At the bottom of the right-click interface, there is an option for Multigraph called “Make Multigraph Overlay”. … Read more »

Layout Editor Ribbon   Layout Editor Tab   Layouts adds, copies, or deletes layouts Iteration groups samples included in the layout batches. For more on Iteration, click here. Batch generates a batch. For more on Batching, click here. File Tab   Document Band Print  opens the Print dialog, for more on printing, click here. Edit Header / Footer opens… Read more »