The contents of any window in FlowJo can be printed. To print the FlowJo Workspace window, select the Print command from the Document band. The Document band, shown in Figure 1, is by default located in the File ribbon. Alternatively, the print command can also be located in the FlowJo Home menu, shown in Figure 2. The FlowJo Home menu can be… Read more »

FlowJo’s interface is based on a Ribbon, similar to that found in Microsoft Office products. This page explains the nomenclature and properties of FlowJo’s implementation of Ribbons. A window’s ribbon is made of thematic tabs, which break into bands, which contain tasks. Ribbons become useful when organizing features in the Workspace, Table, and Layout Editors, which have previously… Read more »

Getting the good stuff inside FlowJo out for other uses! FlowJo makes exporting your analyses quick and easy. From almost every interface of the program there is a way to get your sample data, plots, or statistics out of FlowJo for use in spreadsheets, graphics programs, or almost any secondary software package. The interface areas… Read more »

Command line FlowJo is the way to use FlowJo without a user interface (“headless”) or human intervention. Using command line FlowJo, the user has various options to load data, perform auto-compensation, calculate gates and statistics, generate reports, import CLR files, export populations, and save results. Importantly, users can use command line to execute FlowJo templates… Read more »

The real power of FlowJo is its ability to batch analyses and outputs over many files. Batching, in most simple terms, is creating a list of tasks, putting them in a queue, and sending them to the computer’s CPU to be processed in order. In FlowJo that translates into something as simple as a user… Read more »

FlowJo v10 provides an option to save your analysis, data, and plugin outputs as a single file. An ACS (Archival Cytometry Standard) container file combines your FCS files, the workspace, and plugin outputs into a single file that is similar to a compressed .zip file.  ACS files can be uploaded to a server, sent to… Read more »

Refer to the following external documents for more information regarding the Proliferation platform. Interpretation of Cellular Proliferation Data: Avoid the Panglossian. Mario Roederer, Cytometry Part A � 79A: 95 101, 2011 Assessing Antigen-Specific Proliferation and Cytokine Responses Using Flow Cytometry. Allsopp and Langhorne, Edited by Denise Doolan, Malaria Methods and Protocol, p. 409 Antigen/Mitogen-Stimulated Lymphocyte Proliferation. Whiteside, Measuring… Read more »

FlowJo has several analysis platforms that provide capabilities beyond the gating and statistics used for most simple data analysis.   Workspaces and Demo Data are provided for several of FlowJo’s platforms so that you can try out these features yourself . In addition, we have several short Tech Notes that give step-by-step instructions for FlowJo platforms… Read more »

To obtain a better fitting model, you can fix the location of the undivided population. Click on the curve of the undivided population and drag the center line to place the curve in the optimum position.  The black control handles can be dragged to independently adjust width and height, left and right arrow keys move… Read more »

Question 1: I have a CFSE plot where the majority of the cells are in the peak corresponding to four rounds of division. Yet- the proliferation index is only 2.14.  Although I understand that this is the relevant number and is what people use in the literature, I can’t explain why the proliferation index number is… Read more »