The Layout Editor’s Grid Tool makes it easy to organize your multi-plot layouts by grouping them into a single table. You can use grids for keeping graphs aligned and grouped, or you can model 96 well plates by dropping an entire plate of files onto the first cell of the grid. To create a grid:… Read more »

Edit any plot even after it has been placed into the Layout Editor. After placing plots into the Layout Editor, double clicking on any plot or overlay will bring up the Graph Definition window. From this window many alterations and customization’s of your plot are available. The window has four tabs: Specify, Annotate, Fonts and Legend…. Read more »

To add a header and/or footer to your layout or table when it is printed or output, select Edit Header/Footer from the Document band (for layouts) or the Clipboard band (for tables) .   Check the box in the dialog to have FlowJo include either a header, a footer or both. Standard HTML can be used for rich… Read more »

Using Statistics in FlowJo’s Layout When a statistic is dragged to the Layout Editor, it is displayed in a manner similar to Figure 1, below. Figure 1: Statistics can be dragged from the Workspace (left) to the Layout Editor (right). These statistic objects can be edited. If you double click a statistic object, stylistic changes… Read more »

There are four main steps to create a successful batch in FlowJo: Build a Prototype tile Choose the batch type and destination Check group selection and workspace sort order Run the batch Building a Prototype Tile After performing all your necessary analysis, building a prototype tile is the next step. By building a prototype tile you are… Read more »

Tired of reformatting your layout every time you run an experiment? Format your figures, statistics tables, and accessory objects with the click of a button using FlowJo v10’s Figure Presets.   Figure presets are analogous to a local set of formatting preferences within the Layout Editor. Any graph, legend, statistics table, text box, or object in the… Read more »

From the Layout Editor, graphical layouts can be exported in a variety of formats: – to clipboard (copy/paste) – to file (save or batch-to-file) Related Links: Batch Outputs Copy/Paste of images within FlowJo or to a 3rd party application like PPT (PowerPoint) If taking your data to PowerPoint or Microsoft Office products, the EMF format option… Read more »

Any text box within the FlowJo’s layout editor is capable of evaluating dynamic equations. These may be simple sums or ratios of statistics, or can be arbitrarily complex algebra formulas. To create a text box, simply click on the text tool in the toolbar. You can also just drag a statistic in to a layout… Read more »

In many ways, the Layout Editor is a like a simple page layout program. It can draw boxes and lines, type text and move the items around with a familiar interface. It has some additional aspects as well: it supports multiple pages each with its own layout defined; it has controls to apply the same layout… Read more »

FlowJo vX makes it easy to batch overlays and customize your graphical reports. Batching overlays is a powerful tool that enables you to view any number of control samples overlaid with test samples across an entire experiment. The general process is the same for all overlay batches: create an overlay in a layout, specify (lock)… Read more »