There are 3 lock features in the Layout Editor located on the Objects tab.   Any object, graph, statistic table, or graphic can use the Lock feature. Lock Position: Locks the position of the object. Lock Content: Locks statistics so iteration does not affect values. Locks content in text boxes and any item/object so changes cannot be made… Read more »

The Line/Arrow tool is used for drawing simple lines and arrows in the Layout Editor. Select this tool then click and drag anywhere in your layout to draw a line. To resize it, click on one of the square handles at the ends of the line. To move the line, move the mouse over the line… Read more »

Sample Mismatch!?! Now what? FlowJo can organize a batch by sample, by stain panel, or by keyword. (Complex batches can be organized by a sequence of these parameters.) Set these preferences with the drop-down menus at the top of the layout editor. If batching is set to a particular sample name, and a different sample is… Read more »

SAVE HOURS OF YOUR LIFE – BATCH! One of the strengths of computers is how they handle repetitive tasks. Show the computer how you want something done once, and it can repeat the task a million times without complaint. Flow cytometry often requires application of the same analysis to any number of samples, and FlowJo’s ability… Read more »

The Layout Editor’s Grid Tool makes it easy to organize your multi-plot layouts by grouping them into a single table. You can use grids for keeping graphs aligned and grouped, or you can model 96 well plates by dropping an entire plate of files onto the first cell of the grid. To create a grid:… Read more »

Edit any plot even after it has been placed into the Layout Editor. After placing plots into the Layout Editor, double clicking on any plot or overlay will bring up the Graph Definition window. From this window many alterations and customization’s of your plot are available. The window has four tabs: Specify, Annotate, Fonts and Legend…. Read more »

To add a header and/or footer to your layout or table when it is printed or output, select Edit Header/Footer from the Document band (for layouts) or the Clipboard band (for tables) . Check the box in the dialog to have FlowJo include either a header, a footer or both. Standard HTML can be used for rich text,… Read more »

Using Statistics in FlowJo’s Layout When a statistic is dragged to the Layout Editor, it is displayed in a manner similar to Figure 1, below. Figure 1: Statistics can be dragged from the Workspace (left) to the Layout Editor (right). These statistic objects can be edited. If you double click a statistic object, stylistic changes… Read more »

There are four main steps to create a successful batch in FlowJo: Build a Prototype tile Choose the batch type and destination Check group selection and workspace sort order Run the batch Building a Prototype Tile After performing all your necessary analysis, building a prototype tile is the next step. By building a prototype tile you are… Read more »

Tired of reformatting your layout every time you run an experiment? Format your figures, statistics tables, and accessory objects with the click of a button using FlowJo v10’s Figure Presets.   Figure presets are analogous to a local set of formatting preferences within the Layout Editor. Any graph, legend, statistics table, text box, or object in the… Read more »