FlowJo draws only one type of contour plot, using equal probability contouring. This algorithm generates, in general, graphs which are most accurately interpreted by our brains, in terms of relative frequencies of subpopulations. All contouring algorithms have advantages and disadvantages, but probability contouring is probably the most informative. Combined with the display of outliers, this… Read more »

You worked hard for your data, they deserve to look great! The multitude of plot types in FlowJo’s Graph Window provide ample means for showing off your data in their best form. FlowJo provides several different choices for both bivariate and univariate data displays. The bivariate options can be separated into two broad categories; density plots… Read more »

CDF, or Cumulative Distribution Function plots display exactly the same information as do histograms. The difference is that the histogram values are summed as the fluorescence intensity increases; thus, the CDF begins at 0% (origin) and ends at 100% (maximum Y value). CDF plots are extremely useful for quickly finding the fluorescence of a distribution… Read more »

Click on the Auto gate tool button on a bivariate graph window to activate the auto gate feature. When moving the mouse over the graph, FlowJo will automatically find subpopulations to gate. These are shown on the graph window and change based upon equal probability distributions. This is the same algorithm used for identifying contour lines for… Read more »

Quickly generate graphical reports of all your plots, histograms, plates, and overlays, in a variety of output formats. FlowJo v10 has added features to enhance your graphical output, check your gating strategy, and apply common formats to multiple objects! The Layout Editor is FlowJo’s tool for creating graphical reports. Like other areas of the program,… Read more »

The Text tool will adding additional text to your layout (notation, plot descriptions, etc.). The ability to handle text is greatly improved in FlowJo X. Multiple fonts, styles, colors and sizes can be mixed in the same block of text. Pieces of text can be static, or can be dynamically generated from the properties of… Read more »

Fully batched Tables can be transferred to the Layout editor by choosing to Current Layout from the menu of a generated table and then batching. Once done, the Layout Editor will open, and your table will be added to the layout. *Note: the table will go into the last selected or currently selected layout This is an example… Read more »

Batch processes save you time by allowing you to design a prototype reports (tile) and then tell FlowJo to apply that tile to groups of samples. In the case of a simple batch layout, each tile of the batch corresponds to one sample in a group. A tile is group of objects (plots, statistics tables,… Read more »

The Rectangle tool is used for drawing simple boxes and frames in the Layout Editor. Select this tool then click and drag anywhere in your layout to draw a rectangle. To resize it, click on one of the dark handles at the corners of the rectangle. To move it, mouse over the edge of the… Read more »

Show your work off to the world! Anything in the canvas can be printed from the Layout Editor. This includes samples, tables, and graphics. Printing from the Layout Editor is very simple. 1. Add the samples, tables, and statistics you wish to print to the Layout Editor. 2. Adjust the page breaks by grabbing a corner… Read more »