FlowJo is littered with help buttons throughout the application. Clicking on one of these launches a web browser to access our web page describing that topic. If you need immediate assistance please call us at +1 (541)-201-0022 or (800)-366-6045 or email us at flowjo@bd.com The Help band in the Workspace contains buttons with menus of context-specific links to… Read more »

The Graph Window is the analysis interface that facilitates data visualization and gating. Displaying a Graph: To display a graph of the data, double-click on a file in the Workspace and a plot will appear, which is called the Graph Window. Visualizing the Data: There are several different kinds of plots that can be used to display… Read more »

FlowJo v10 presents: The Zebra Plot Bivariate zebra plots are a hybrid plot type; a mixture of both contour and density plots. They are computed exactly the same way as are contour plots (equal probability contouring) but mixed with a color gradient that spans each contour area. This provides an additional visual cue to event… Read more »

Biexponential scaling helps visualize data that is compressed against the low x- and y- axes. “Squished” data is easily viewed by adding a section of linear scale to log acquired data. Alternate display transformations are intended to provide a more intuitive view of flow cytometry data.  Specifically, they address the problem of how to visualize… Read more »

FlowJo v10 makes it easy to transform your raw data to expand or compress what is displayed on a graph. The transform does not modify your actual recorded fluorescence data, only the amount of visual space that is allotted to various regions of the data. All digital (fcs3.0) data is output as linear and the… Read more »

Did you acquire your data with BD’s FACSDiva software? Compensate during acquisition? No problem! FlowJo can read FACSDiva data files and apply the compensation matrix you used at the time of acquisition automatically. Export from Diva Current BD FACSDiva version 6.x can export data two ways;  “Export Experiment” or “Export FCS files”.  We highly recommend… Read more »

Transform digital data quickly and easily in FlowJo using the transform function on the graph window. Flow cytometers collect analog information and convert it to digital information via hardware and software components during acquisition. The mathematics behind this conversion is complicated but can be reduced to the amount of resolution a cell or “event” has… Read more »

Data visualization is important in flow cytometry data analysis – transformation provides a method to visualize populations in a shape that’s easier to interpret by eye, because they resemble normal distributions. Traditionally, without biexponential transformation, after background fluorescence subtraction and the introduction of compensation error, data points may have negative fluorescence. In a standard log scale,… Read more »

A tethered gate derives its position from a statistical operation. In  FlowJo a range gate in a histogram can be dynamically positioned by a function of the data itself.   The tether gate feature makes it possible to set a gate dependent on a statistic and have that gate adjust from sample to sample when applied… Read more »

Use the T-button to quickly adjust data scaling and visualization. Data visualization is key to identifying and gating different populations within a sample or experiment. Have you ever noticed how “cells” (events) pile up on the left or bottom axes? Perhaps you have antigen positive subsets that are only marginally brighter than controls? The “T” or “Transform” button provides… Read more »