Select the Tables button in Preferences under Output to open the Tables specific preferences. Tables Export Format: Choose the output format used most and set it as the default. Like Excel. Open After Saving: If checked, output file will be open after saved. Close Tables Without Save: If checked, tables will be disposed upon closing without asking you to save…. Read more »

To reset Preferences to factory default: Open Preferences by clicking on the heart icon in the upper right corner of FlowJo. Figure 1. The Heart Icon. Use the Reset button in the lower left hand corner to set the configuration file for FlowJo to its default settings. Click ok in the confirmation dialog to confirm reset… Read more »

Ranges  predefined and named minimal and maximum to automatically format statistics out of expected ranges in the table editor. Ranges can be set in Preferences, under the Tools section. Ranges are predefined for ease but are completely editable for customization. The two button at the top allow you to add a new range or delete an… Read more »

FlowJo Preferences can store your preferred starting point for specialized platform analysis. This includes Cell Cycle  and Proliferation! Fig 1: The Cytometry Platforms Preferences panel Cell Cycle preferences allow setting of the default model that Cell Cycle nodes should be created with – Watson vs DJF. You can also control aesthetic details such as which layers of the… Read more »

Select the Performance button in the Preferences tool to open the Performance preferences.  If you have experienced an “out of memory error,” please scroll down to “Memory Management,” below. GUI (Java) Threads This integer tells FlowJo how many threads the Graphical User Interface should open at the start of the program. This preference requires that the program… Read more »

Select the License button in Preferences to open the License preferences. The License preferences has been broken down into sections with detailed descriptions below. FlowJo Portal Access: Email: Lists the last portal email that was used. Show Remember Me: Ticking this will show the option when logging into portal to remember your user name and password so you… Read more »

Select the Layouts button in the Preferences window to open the Layouts-specific preferences. Layout Options Batch Format: This specifies where a new batch will be output; applies to new layouts in new workspaces. A batch report can be exported to a new layout, or to several other export options. Default Zoom: Choose the default zoom for newly opened layouts. Iteration Type: Select Sample from this… Read more »

Setting Local in the Workspace The “Locale” option can be chosen from the preferences under the heart icon. The Local preference will control a few things specific to the region you are using FlowJo in. Mainly it will determine what order the date will appear in and it will determine wether a comma or dot… Read more »

Graphs button in the Preferences tool to open the Graphs preferences. Descriptions are broken into sections below. Graph Type Graph Type : Set the default graph type for all newly created graphs. Contour Levels : Set the percentage of cells that each contour line represents, or switch to logarithmic scaling of the contour lines. Smooth : If checked the spaces between… Read more »

FlowJo supports the notion of a global preferences file. If your workstation is shared among multiple users, you can place a single global prefs file with the serial number in one place, while all other individual prefs will be stored in the users’ individual My Documents folder. FlowJo global preferences file should be placed either… Read more »