Gating/Gates

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To view or change your gating preferences, click the preferences icon represented by a red heart outline on the workspace toolbar. Once you open the preferences window, select Gates and you will see the window below Gate Settings: The gate settings determine the default appearance of gates in graphs.  You can set gates to tinted, change gate… Read more »

Font and Text

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Select the Fonts button in the Preferences tool to open the Fonts specific preferences. Choose a Context where you would like to set your font preferences, then change the Text Settings for that context. Look in the Sample Text area to get a preview of the font. When you have found your desired font, click OK. Context Section: Select the context to… Read more »

File Formats and Data Access

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Select the File Formats button in Preferences under Output to open the File Formats specific preferences. Formats Use these drop-down menus to specify the default file format for FlowJo tools. Your choice will depend on the target of your output. Click here for more details on output. Graph Window: Select the default graphic format for export from the Graph… Read more »

Diagnostics

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Select the Diagnostics button in Preferences to open the Diagnostics specific preferences. Settings Open Engine Interaction Window: If checked, FlowJo will start with the engine interaction window; may sometimes be necessary to troubleshoot network licensing related problems. Local Website: Enter URL for local help access. Local Contact: Enter your email address for replies from Technical Support. Write default… Read more »

Cytometer Preferences

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FlowJo version 10 allows you to set up scaling preferences based on the specific cytometer being used. We have a number of default settings for the various cytometers set.  However, these can be changed or refined. For more information about FlowJo’s display of data for specific cytometers, please see FlowJo and Your Cytometer. Background Data is written… Read more »

Compensation

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Set your default preferences for compensation Select the Compensation button in the Preferences tool to open the Compensation preferences. The interface will open as shown below: Compensation Group Inclusion The “Group Inclusion” can be used to make files with certain file names get automatically included in the group when they are loaded into FlowJo. $FIL – is the… Read more »

Annotation, Legend and Extras

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Select the Layout Annotation button in the Preferences tool to open the Layout Annotation preferences. Annotation Use Annotations This setting enables/disables annotations in layouts. Show Graph Title: In the graph annotation, show the title of the graph. Show Sample Name: In the graph annotation, show the sample name. Show Frequency: In the graph annotation, show the event frequency… Read more »

Installation

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To install FlowJo, please download the installer from our website at: http://www.flowjo.com You can download the installer appropriate for your platform (Mac or PC), by visiting the Download tab there. PC Download the installer.exe file and double click on it to install the program. Simply press the “Next” button to agree to the default settings in the… Read more »

Installation From the Web

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Getting FlowJo on your computer has never been easier, faster, or more convenient! Simply download and install from our website. If you don’t yet have a license, simply sign up for a FREE 30 day trial. Analyze your own data or play with our tutorials. Watch our video on this topic! Download FlowJo v10 FlowJo… Read more »

Getting Started and FlowJo Licensing Information

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First, you need to install FlowJo by downloading the installer from our website. If you have already installed FlowJo, you may skip to the Initial Launch, License Information, and License sections of this document below.  PC and Mac Installers You can select your platform (Mac or PC) at the top of the page. For a PC, download the .exe… Read more »