FlowJo allows you to add new parameters to your data by performing operations on existing parameters. We call these “Derived Parameters“.

Once created, they behave like parameters that were collected at acquisition. They can be used in the graph window, or in plots within the layout editor.

To open the Derived Parameters interface, click the Workspace tab (if running FlowJo in Experienced mode) or Tools tab (if running FlowJo in Advanced mode) > Instrumentation to locate the Derived Parameters button. Or right click on the sample in the Workspace and select Derived Parameters.

For more on Derived Parameters, click here.