Partec uses FloMax software on most cytometers, which produces FCS 3.0 compliant files. FloMax rescales the fluorescent data on the acquisition display without changing the stored values. FlowJo plots the stored values.
Preferences in FlowJo are set by default to scale the data correctly according to the acquisition display and the format of the file. You can change these default preference sets in the cytometer specific preferences.
Manual adjustments can also be made after data load by following these instructions:
FlowJo instructions for formatting your plots to match FloMax
- Drag and drop the data into FlowJo.
- Because FloMax rescales the data for display and FlowJo does not, the scales will be different, but the data will look the same. Because the scale of flow data is relative, this is acceptable
Below are examples of data as it appears in FloMax and the same data in FlowJo