CBA Demo Video
Below is a demo showing the use of the CBA plugin
Install and Run
Place the plugin JAR file into your plugins folder. Restart FlowJo, load CBA standards and test samples into a workspace. Select a sample and run the CBA plugin.
Choose the standards to use for calibration of analytes.
Check the beads populations auto-gated in FlowJo by scrolling down the list.
Note: To make adjustments double-click on the plot of FSC vs SSC.
Choose from a custom kit of anaytes in your CBA kit, or customize the list using the “Custom” tab there:
Select clustering parameter(s) (toggle 2D to add a secondary clustering parameter), and a reporter parameter:
Note: If necessary, double-click on the graph to adjust gating location/geometry. Researchers can scroll through the Samples list at the top of this dialog to confirm the clustering performed on each sample.
Enter concentrations for standards in list of reagents – List can be ordered by the MFI of reporter parameter in clusters in order to utilize the dilution option:
Confirm regression performed for each analyte:
Generate outputs in FlowJo, including: Static images generated for the regression to estimate analyte concentrations, derived parameters created in each sample from the regression to estimate true analyte concentrations, and pre-made tables to calculate the MFIs of those same analytes.