Keywords used in the FlowJo workspace, are valuable annotation embedded in FCS data files. You can utilize them to sort and filter samples based on their annotation characteristics. These keywords are combined with groups to effectively subset analyses into components. Add Keyword Clicking this icon allows the user to create a new attribute/value pair for… Read more »

The Experiment band allows users to include additional information in an analysis Add Keyword Click on that button to add additional keyword-value pairs to the Workspace Annotate Experiment This dialog supports the new conventions for labeling metadata covered by MIFlowCyt. Plate Editor Access the Plate Editor. The Plate Editor can annotate FCS files from plate-based assays.

Selecting an Expand/Collapse icon provides four actions to manipulate the display of a Workspace by expanding or collapsing the analysis hierarchy of a particular sample or a group of samples. These functions reduce scrolling when you are analyzing a large number of samples. Expand Selection Reveals any hidden items in the analysis hierarchy of the selected population… Read more »

There are two action icons located within the Find band that allow you to search for FCS data files or text within these data files. These actions can help you quickly locate and/or replace keywords and files from your Workspace. Find Search for text in keywords of the data files. FCS Scan Process your local… Read more »

From the FlowJo Navigate band, users can open FlowJo’s Table Editor and Layout Editor. This band also allows the user to create a new workspace, access preferences, add a new group to the existing workspace, or browse the file system for samples (in .FCS format) to add to the current workspace. New Workspace Clicking this icon will… Read more »

FlowJo’s Biology Band contains a series of tools for applying mathematical models to specific biological systems. Cell Cycle The cell cycle platform creates a one-dimensional model of DNA content for the selected population using either the Watson or Dean-Jett-Fox models. To use this tool, click on a population at any level of the gating hierarchy… Read more »

The Boolean gates ribbon contains a series of tools for using existing gates to create additional, more complex gates. Create Combination Gates Create Combination Gates makes a set of Boolean gates comprised of all possible combinations of the gates selected at the time this function is chosen. For example, if you have gates “a”, “b” and… Read more »

The Clipboard band contains functions that allow the user to duplicate or remove samples, statistics, or pieces of text from the Workspace. The most recently cut or copied item is saved to the clipboard and can be inserted back into the workspace using the Paste function. Cut The Clipboard band contains functions that allow the… Read more »

The Power band provides access to diagnostic and benchmarking functions. This should not be required for routine use of the program, but is helpful if attempting to resolve problematic cases.  Diagnostics Contains access points to: Script editor Allows users to code additional functionality into FlowJo within the program.  Uses Java Script.

The Document band allows the user to save changes to the workspace, revert to the last save state, or export the workspace to the user’s hard drive. It also contains functions for renaming and inspecting selected samples from the workspace and opening the graph windows for those samples. Print Clicking this icon opens a dialog… Read more »