Monitor FlowJo’s Progress on Tasks – or use it to dock interesting URL’s FlowJo’s Workspace window is loaded with many controls, including the bottom edge of the window: Most of the time you’ll see activity in that area when FlowJo is busy working on large jobs – as a progress indicator. Any error messages pertinent… Read more »

Stratedigm uses FCM software on the SE500 and S1000 machines, which produces FCS 3.0 compliant files. Preferences in FlowJo are set by default to rescale the data based on our understanding of the differences between their acquisition display and the written format of the file.  FlowJo’s default preferences should display the data the same way… Read more »

Partec uses FloMax software on most cytometers, which produces FCS 3.0 compliant files.  FloMax rescales the fluorescent data on the acquisition display without changing the stored values.  FlowJo plots the stored values. Preferences in FlowJo are set by default to scale the data correctly according to the acquisition display and the format of the file…. Read more »

Miltenyi cytometers use MACSQuantify software, which produces both MQD and FCS 3.1 files. The MACSQuantify rescales the data in the acquisition on a hlog display without changing the stored values.  FlowJo displays the stored values. Preferences in FlowJo are set by default to rescale the data based on our understanding of the differences between their… Read more »

InCyte Files Millipore’s Guava cytometers use InCyte software, which produces FCS 3.0 compliant files.  InCyte can export a set of data files as either a single multi-run FCS file, or individual files.  FlowJo works best with the individual files. Preferences in FlowJo are set by default to rescale the data based on our understanding of the… Read more »

  SONY use similar acquisition software on the EC800 and SH800 cytometers. Both produce FCS 3.0 compliant files. However, Eclipse SW rescales the data in the acquisition display without changing the stored values.  FlowJo v10 rescales the data based on its understanding of the acquisition display. The SONY SY3200 uses WinList for acquisition. There is no difference… Read more »

Cytek uses SpectroFlow for the Auroa line of spectral cytometers, and Cypod software for older products, which both produce FCS 3.0 compliant files.  Please contact us with any problems.

FlowJo v10 preferences allow you to control/modify the data display of Coulter files. Don’t fret if the data looks different in FlowJo than on the machine. It CAN BE fixed! CytoFLEX platform These instruments use CytExpert software, which produces FCS 3.0 compliant files. Click here for more information. FC500 and Gallios/Navios These instruments produce LMD files… Read more »

Analog instruments such as BD FACSCalibur and BD FACScan produce FCS2.0 files. Analog data (or FCS2.0) data files are read by FlowJo in the format they were exported.  Analog data files are exported with data scaling information and compensated fluorescence values (if compensation was done on the instrument).  Therefore, all FCS2.0 data read into FlowJo will appear exactly*… Read more »

FlowJo can read FCS files from any BD Cytometer and can import/export Diva workspaces The following BD cytometers use BD FACSDiva software for acquisition: (BD FACSCelesta, BD FACSCanto II & I and 10-color BD FACSAria I, II, and III BD LSR I and II BD LSRFortessa BD LSRFortessa X-20 BD FACSSymphony A1, A3, A5 and… Read more »