First, you need to install FlowJo by downloading the installer from our website. If you have already installed FlowJo, you may skip to the Initial Launch, License Information, and Serial Number sections of this document below.


 PC and Mac Installers

You can select your platform (Mac or PC) at the top of the page. For a PC, download the .exe file and open it to install the program. Choose to place a shortcut on the desktop, and when the installer finishes, double-click on the FlowJo shortcut icon to launch the program.  For a Mac, download the .zip file and double-click it to extract the program. Once the zip file is extracted, double-click on the icon to launch FlowJo.  For more information on installation please see our pages describing installation from the web and from a CD.


Obtaining a FlowJo License

You have downloaded the full version of FlowJo.  However, until a serial number or dongle is provided, FlowJo will only load specially enabled demonstration data files, such as those provided with our tutorials.  For more information on obtaining a FlowJo license, please see our license options page or submit a request for a quote.   To obtain a trial 30-day serial number so that you may try FlowJo with your data please fill out the form on our 30-day trial serial number page.


FlowJo Version 10.0.7

We encourage you to update to the most recent version of FlowJo, and if you are launching FlowJo with a version number 10.0.6 or earlier, please see the directions below for navigating the Welcome and License screens.  When FlowJo is launched for the first time, the FlowJo license information window will open with the licensing agreement and license options.  First, click on the checkbox to accept the license terms.


Figure 1. Accepting the License Terms.


Next, if you have a dongle (a physical thumb drive that has a license key on it) plugged into your computer, FlowJo will detect it automatically and allow you to click “Done” to authenticate (Figure 2).  If you do not have a dongle plugged into a USB drive on your computer, have an individual license serial number, or are a member of a site license, then you will need to enter your serial number as shown in Figure 3.  If a valid 16-character serial number is entered, no warning message will be displayed and you may click “Done” to begin using FlowJo.  If there is an authentication error, a warning message will appear; please double-check the serial number to ensure it has been transcribed correctly, and the hardware address to ensure the correct serial number is being entered for the corresponding hardware address.


Figure 2. Automatic detection of a dongle.


Figure 3. Entering a Serial Number.


“Join an enterprise group license” is for FlowJo Enterprise sites.  For more information please contact  “Continue under free demonstration license” will allow you to only load and analyze specially enabled demonstration data files, such as those provided with our tutorials.


See Also:

Tutorials to Download

FlowJo University

FlowJo Webinars