FlowJo’s 3D Tool displays three parameters of your FCS data simultaneously. The 3D viewer can be accessed from many different areas in FlowJo.
Both of these options use whatever parameters was viewed in the Graph Window last. The default is FCS-A on the x axis, SSC-A on the y axis, and FSC-W on the z axis.
From the right-click menu in the Workspace
From the Tools Tab in the Worksapce
From the Graph Window
From the Table Editor. Must have 3 statistics selected and the 3 statistics are place on axes x, y, and z.
The 3D viewer looks like this when opened. There several options in the platform.