The Bivariate Cell Cycle platform produces population statistics for each of the three main cell cycle populations (G1, S, and G2), as well as for hypoploid and hyperploid cells. This page is under construction; stay tuned for FlowJo Version 10 with Cell Cycle.

The Bivariate Cell Cycle platform identifies G0/G1, S, G2/M, hypoploid, and hyperploid populations within the data. For an explanation about how the model works, see the help page on the Bivariate Cell Cycle model. For each of those populations, FlowJo reports:

  • A bivariate Median Fluorescent Intensity (MFI) of the two parameters used, typically a measure of DNA content like PI and an S-phase determinant like BrdU.
  • The percent of events assigned to each population relative to the parent population.

 

 

Figure 1

These statistics are placed in a table within the model window, with the colors and labels matching the graphic portion of the model window as shown in Figure 1. The statistics also appear in the workspace automatically as well, as shown in Figure 2. The statistics will update if the user manually adjusts the gates created by the model in the cell cycle platform window. Additionally, the bivariate model automatically creates a node for each population in the Workspace. You may add any additional statistic in the traditional manner through the Statistics band.

 

Figure 2

For more information see the relate links listed below:

Cell Cycle Models

Univariate Cell Cycle

Bivariate Cell Cycle

Multivariate Cell Cycle

Cell Cycle Preferences

Cell Cycle Protocols and Papers

Protocol for DNA / Cell Cycle using PI and BRDU

List of References about DNA dyes and analysis

Download a Cell Cycle Workspace with Demo Data to try out this platform