The Multigraph feature in version 10 is an excellent data mining tool for exploring your data in N dimensions.  The Multigraph feature can be used in the layout editor with data that has not been overlayed with any other sample or population.  However, the real power of Multigraph is evident when overlaying multiple samples or populations.

To access the Multigraph interface in version 10, place a plot in the layout editor and right click on the plot. (As stated above, the plot can be of a single population/sample or of an overlay of multiple samples/popualtions.  The following examples will all be demoed with overlaid populations, but remember that the Multigraph function can be utilized with plots that have not been overlaid.)

At the bottom of the right-click interface, there is an option for Multigraph called “Make Multigraph Overlay”:

If you expand the Multigraph interface, you’ll notice three options:

  1. Histograms will provide you a multigraph with a histogram of every parameter in the data file
  1. All Parameters by Y will provide a multigraph with every parameter versus the currently displayed Y axis of the initial plot
  1. NxN Plots with display every parameter versus every other parameter in the data file

As you can see, Multigraph is a great data mining/exploration tool.  Multigraph is a useful option for viewing all your parameters, particularly when using an overlay.  Visualizing changes in fluorescence in N dimensions can be very beneficial in order to properly interpret the data.

One final note: When you create a multigraph, all parameters will always be displayed by default.  However, if you right click on the multigraph, you are presented with an option to remove any parameter(s) from the display.  Use this feature to limit the number of parameters complicating the analysis!  This functionality can be utilized in all three types of multigraphs.